caa a22 4500 44585801X CHVBK 20180317145434.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10592-011-0236-7 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0236-7 Phylogeographic analysis reveals two cryptic species of the endangered fern Ceratopteris thalictroides (L.) Brongn. (Parkeriaceae) in China [Elektronische Daten] [Yi-Ying Liao, Xing-Yu Yang, Timothy Motley, Jin-Ming Chen, Qing-Feng Wang] Ceratopteris thalictroides (L.) Brongn. (Parkeriaceae) is a difficult fern species to taxonomically classify. Three cryptic species were revealed in the previous studies, referred to as the north type, the south type, and the third type. Because much of the distribution range of C. thalictroides in China was not included in the sampling of the previous studies, the taxonomic complexity of C. thalictroides in China remained uncertain. In order to identify the uncharacterized cryptic species, we examined four chloroplast DNA (cpDNA) non-coding regions and compared sequence variation within this species complex. Sequence data were obtained from 143 individuals in 24 populations throughout the natural distribution of the species in China. Nineteen haplotypes were identified. Molecular systematic and phylogeographical analyses revealed two genetically distinct clusters of cpDNA haplotypes in China. One cluster included haplotypes associated with the north type, and another with the south type cryptic species. The N ST value was significantly higher than the G ST value (N ST=0.768>G ST=0.434, P<0.05), indicating the presence of a significant phylogeographical structure of C. thalictroides in China. The results of AMOVA analysis showed a significant inter-group differentiation (F ST=0.918; P<0.001). Analyses based on different, but complementary methods suggest that in China, C. thalictroides contains only two of the cryptic species (the north and south types). Two haplotypes, H8 and H17, of the interior node in the minimum-spanning network (MSN) of cpDNA haplotypes are widespread. The origin of the widespread haplotypes in China may have resulted from long-distance dispersal to China. Springer Science+Business Media B.V., 2011 Ceratopteris thalictroides nationallicence cpDNA non-coding region nationallicence Cryptic species nationallicence Fern nationallicence Phylogeography nationallicence Liao Yi-Ying Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, People's Republic of China aut Yang Xing-Yu Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, People's Republic of China aut Motley Timothy Department of Biological Sciences, Old Dominion University, 23529-0266, Norfolk, VA, USA aut Chen Jin-Ming Key Laboratory of Aquatic Botany and Watershed Ecology, the Chinese Academy of Sciences, 430074, Wuhan, Hubei, People's Republic of China aut Wang Qing-Feng Key Laboratory of Aquatic Botany and Watershed Ecology, the Chinese Academy of Sciences, 430074, Wuhan, Hubei, People's Republic of China aut Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1357-1365 1566-0621 12:5<1357 2011 12 10592 https://doi.org/10.1007/s10592-011-0236-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0236-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao Yi-Ying Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Yang Xing-Yu Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Motley Timothy Department of Biological Sciences, Old Dominion University, 23529-0266, Norfolk, VA, USA aut NATIONALLICENCE 700 1- Chen Jin-Ming Key Laboratory of Aquatic Botany and Watershed Ecology, the Chinese Academy of Sciences, 430074, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Wang Qing-Feng Key Laboratory of Aquatic Botany and Watershed Ecology, the Chinese Academy of Sciences, 430074, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1357-1365 1566-0621 12:5<1357 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer