caa a22 4500 445858117 CHVBK 20180317145434.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10592-010-0156-y doi (NATIONALLICENCE)springer-10.1007/s10592-010-0156-y Genetic structure of the critically endangered plant Tricyrtis ishiiana (Convallariaceae) in relict populations of Japan [Elektronische Daten] [Hiroaki Setoguchi, Yuki Mitsui, Hajime Ikeda, Naofumi Nomura, Atsushi Tamura] Tricyrtis ishiiana is a relic endemic plant taxon of the Convallariaceae that inhabits two nearby gorges in Kanagawa Prefecture, Japan. The distribution range and number of populations are thought to have been reduced to the present refugial populations during the Quaternary climatic oscillations. Because of its showy flowers, this plant has faced illegal removal from its natural habitats for horticultural use and has been designated a critically endangered species (class IA). In this study, we analyzed the genetic structure of the relict populations of T. ishiiana in order to contribute to the conservation strategies of the prefectural government. Our analyses of nine nuclear microsatellite loci detected high genetic diversity (HE=0.704 and HO=0.541) for the two populations. The two populations were slightly differentiated (RST=0.032), accompanied by faint substructure across the populations (K=3). In addition, each population exhibited spatial genetic structuring. The relatively low inbreeding coefficient for both populations together (FIS=0.233) and each population separately (FIS=0.217-0.246) may be attributable to crossing among descendants within a population along with occasional gene flow between the populations. These results suggested that the extant populations have not experienced a severe bottleneck. The two extant populations were genetically differentiated at a very low level, accompanied by occasional pollen flow via pollinators and/or seed dispersal by gravity in the mountainous environment. Occasional gene exchange between the populations has allowed T. ishiiana to harbor high genetic diversity despite being a relic plant confined to two small refugial populations. Springer Science+Business Media B.V., 2010 Convallariaceae nationallicence Gene flow nationallicence Microsatellite nationallicence Refugia nationallicence Tricyrtis ishiiana nationallicence Setoguchi Hiroaki Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut Mitsui Yuki Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut Ikeda Hajime Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut Nomura Naofumi Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut Tamura Atsushi Kanagawa Prefecture Natural Environment Conservation Center, Nanasawa 657, 243-0121, Atsugi, Japan aut Conservation Genetics Springer Netherlands 12/2(2011-04-01), 491-501 1566-0621 12:2<491 2011 12 10592 https://doi.org/10.1007/s10592-010-0156-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-010-0156-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Setoguchi Hiroaki Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Mitsui Yuki Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Ikeda Hajime Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Nomura Naofumi Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Tamura Atsushi Kanagawa Prefecture Natural Environment Conservation Center, Nanasawa 657, 243-0121, Atsugi, Japan aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/2(2011-04-01), 491-501 1566-0621 12:2<491 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer