caa a22 4500 445858281 CHVBK 20180317145435.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10592-010-0146-0 doi (NATIONALLICENCE)springer-10.1007/s10592-010-0146-0 The genetic status of two subspecies of Rhodeus atremius , an endangered bitterling in Japan [Elektronische Daten] [Takuya Miyake, Jun Nakajima, Norio Onikura, Shigetoyo Ikemoto, Kei'ichiro Iguchi, Akira Komaru, Kouichi Kawamura] Rhodeus atremius is an endemic bitterling fish from Japan comprising two endangered subspecies, R. a. atremius and R. a. suigensis. The latter subspecies, which had dramatically declined in last decades, was reported to contain two distinct mtDNA lineages. In order to estimate the optimized units for conservation management, the genetic structure of R. atremius was inferred by analyzing mtDNA and microsatellite markers (MS). Allelic richness and heterozygosity of MS in R. a. suigensis was less than half that in R. a. atremius. In R. a. suigensis, not only within-population genetic diversity but also among-population genetic divergence was low, with the exception of population Ah1, while the diversity was high and the divergence showed isolation by distance in R. a. atremius. In mtDNA and MS, R. a. suigensis concordantly formed a single lineage, while R. a. atremius encompassed four mtDNA lineages, two of which were completely admixed into one group on the basis of MS. In population Ah1 a striking introgression between the two subspecies was suggested by a Bayesian-based assignment test, with the presence of mtDNA haplotype of R. a. atremius. Contrary to the prevailing theory, R. a. suigensis corresponds to a single conservation unit, while three units seem appropriate for R. a. atremius. In addition, low genetic diversity of R. a. suigensis might have arisen mainly as a result of recent bottlenecks before population fragmentation, followed by current anthropogenic effects. Genetic introgression in population Ah1 was probably the result of human transplantation of R. a. atremius. Springer Science+Business Media B.V., 2010 Bottleneck nationallicence Evolutionary Conservation Unit nationallicence Genetic diversity nationallicence Introgression nationallicence Metapopulation nationallicence Rhodeus atremius nationallicence Miyake Takuya Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, 514-8507, Tsu City, Mie, Japan aut Nakajima Jun Faculty of Engineering, Kyushu University, 744 Motooka, 819-0395, Fukuoka City, Fukuoka, Japan aut Onikura Norio Fishery Research Laboratory, Kyushu University, 2506 Tsuyazaki, 811-3304, Fukutsu City, Fukuoka, Japan aut Ikemoto Shigetoyo Okayama Prefecture Environmental Conservation Agency, 665-1 Uchio, 701-0212, Okayama City, Okayama, Japan aut Iguchi Kei'ichiro National Research Institute of Fisheries Science, Fisheries Research Agency, 386-0031, Ueda City, Nagano, Japan aut Komaru Akira Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, 514-8507, Tsu City, Mie, Japan aut Kawamura Kouichi Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, 514-8507, Tsu City, Mie, Japan aut Conservation Genetics Springer Netherlands 12/2(2011-04-01), 383-400 1566-0621 12:2<383 2011 12 10592 https://doi.org/10.1007/s10592-010-0146-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-010-0146-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyake Takuya Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, 514-8507, Tsu City, Mie, Japan aut NATIONALLICENCE 700 1- Nakajima Jun Faculty of Engineering, Kyushu University, 744 Motooka, 819-0395, Fukuoka City, Fukuoka, Japan aut NATIONALLICENCE 700 1- Onikura Norio Fishery Research Laboratory, Kyushu University, 2506 Tsuyazaki, 811-3304, Fukutsu City, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ikemoto Shigetoyo Okayama Prefecture Environmental Conservation Agency, 665-1 Uchio, 701-0212, Okayama City, Okayama, Japan aut NATIONALLICENCE 700 1- Iguchi Kei'ichiro National Research Institute of Fisheries Science, Fisheries Research Agency, 386-0031, Ueda City, Nagano, Japan aut NATIONALLICENCE 700 1- Komaru Akira Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, 514-8507, Tsu City, Mie, Japan aut NATIONALLICENCE 700 1- Kawamura Kouichi Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, 514-8507, Tsu City, Mie, Japan aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/2(2011-04-01), 383-400 1566-0621 12:2<383 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer