caa a22 4500 445863625 CHVBK 20180317145451.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10453-010-9171-1 doi (NATIONALLICENCE)springer-10.1007/s10453-010-9171-1 Exposure to airborne microorganisms, hyphal fragments, and pollen in a field of organically grown strawberries [Elektronische Daten] [Kira Tendal, Anne Madsen] Many working environments are predisposed for larger than average amounts of fungi and other microorganisms often due to organic material being handled. From 2003 to 2007, the area used for strawberry production in Denmark increased by 62%. The purpose of this study was to determine the levels of exposure to microorganisms, endotoxin, (1→3)-β-d-glucan (β-glucan), and pollen in a field of strawberries. The study was carried out in eastern Denmark from the middle of June to the beginning of August 2008. The strawberries were grown organically, and microbiological pest control agents (MPCAs) were applied during this and former growth seasons. In order to measure exposure to inhalable bioaerosol components, we used stationary filter samplers. Bioaerosol sampling was performed during 4 working days, and a total of 57 samplings were performed. The filters were analysed for contents of fungi, MPCAs, endotoxin, β-glucan, and pollen. The mean exposure was 6,154CFU Cladosporium sp. m−3, 1.0×105 fungal spores m−3, 4.1×104 hyphal fragments m−3, 5.8×103 pollen m−3, 57.3ng β-glucan m−3, and 8.9 endotoxin units (EU) m−3. A significant and positive correlation was found between β-glucan and fungal spores and between CFU of Cladosporium sp. and CFU of fungi. We selected specifically for Metarhizium anisopliae, Beauveria bassiana, and the applied MPCAs Trichoderma harzianum, T. polysporum, and Bacillus thuringiensis but found none of these species. In conclusion, our study shows that berry pickers in this organic strawberry field were potentially subjected to higher levels of fungal spores, Cladosporium sp., hyphal fragments, pollen, and thus also β-glucan than is usually seen in outdoor air. Exposure to MPCAs was not seen. The exposure to endotoxin was only slightly higher than e.g. in a town. Springer Science+Business Media B.V., 2010 Allergens nationallicence Biological control nationallicence Fungi nationallicence Bioaerosols nationallicence Horticulture nationallicence Cladosporium nationallicence Working environment nationallicence Tendal Kira The National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen Ø, Denmark aut Madsen Anne The National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen Ø, Denmark aut Aerobiologia Springer Netherlands 27/1(2011-03-01), 13-23 0393-5965 27:1<13 2011 27 10453 https://doi.org/10.1007/s10453-010-9171-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10453-010-9171-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tendal Kira The National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen Ø, Denmark aut NATIONALLICENCE 700 1- Madsen Anne The National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen Ø, Denmark aut NATIONALLICENCE 773 0- Aerobiologia Springer Netherlands 27/1(2011-03-01), 13-23 0393-5965 27:1<13 2011 27 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer