caa a22 4500 445863668 CHVBK 20180317145451.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10453-010-9173-z doi (NATIONALLICENCE)springer-10.1007/s10453-010-9173-z Observations on the use of membrane filtration and liquid impingement to collect airborne microorganisms in various atmospheric environments [Elektronische Daten] [Dale Griffin, Cristina Gonzalez, Nuria Teigell, Terry Petrosky, Diana Northup, Mark Lyles] The influence of sample-collection-time on the recovery of culturable airborne microorganisms using a low-flow-rate membrane-filtration unit and a high-flow-rate liquid impinger were investigated. Differences in recoveries were investigated in four different atmospheric environments, one mid-oceanic at an altitude of ~10.0m, one on a mountain top at an altitude of ~3,000.0m, one at ~1.0m altitude in Tallahassee, Florida, and one at ~1.0m above ground in a subterranean-cave. Regarding use of membrane filtration, a common trend was observed: the shorter the collection period, the higher the recovery of culturable bacteria and fungi. These data also demonstrated that lower culturable counts were common in the more remote mid-oceanic and mountain-top atmospheric environments with bacteria, fungi, and total numbers averaging (by sample time or method categories) <3.0 colony-forming units (CFU) m−3. At the Florida and subterranean sites, the lowest average count noted was 3.5 bacteria CFUm−3, and the highest averaged 140.4 total CFUm−3. When atmospheric temperature allowed use, the high-volume liquid impinger utilized in this study resulted in much higher recoveries, as much as 10× greater in a number of the categories (bacterial, fungal, and total CFU). Together, these data illustrated that (1) the high-volume liquid impinger is clearly superior to membrane filtration for aeromicrobiology studies if start-up costs are not an issue and temperature permits use; (2) although membrane filtration is more cost friendly and has a ‘typically' wider operational range, its limits include loss of cell viability with increased sample time and issues with effectively extracting nucleic acids for community-based analyses; (3) the ability to recover culturable microorganisms is limited in ‘extreme' atmospheric environments and thus the use of a ‘limited' methodology in these environments must be taken into account; and (4) the atmosphere culls, i.e., everything is not everywhere. US Government, 2010 Bacteria nationallicence Fungi nationallicence Methods nationallicence Membrane filtration nationallicence Liquid impingement nationallicence Aeromicrobiology nationallicence Microbiology nationallicence Griffin Dale US Geological Survey, Geologic Discipline, 2639 North Monroe Street, Suite A200, 32303, Tallahassee, FL, USA aut Gonzalez Cristina University of La Laguna, University Institute of Tropical Diseases and Public Health, Avda. Francisco Sanchez, 38271, La Laguna, Tenerife, Canary Islands, Spain aut Teigell Nuria University of La Laguna, University Institute of Tropical Diseases and Public Health, Avda. Francisco Sanchez, 38271, La Laguna, Tenerife, Canary Islands, Spain aut Petrosky Terry US Geological Survey, Water Resources Discipline, 2639 North Monroe Street, Suite A200, 32303, Tallahassee, FL, USA aut Northup Diana University of New Mexico, Biology, MSC03 2020, 1 University of New Mexico, 87131, Albuquerque, NM, USA aut Lyles Mark US Navy, Research Program Integration and Mission Development, Bureau of Medicine and Surgery, 2300 East Street, NW, Bldg 5, 20372-5300, Washington, DC, USA aut Aerobiologia Springer Netherlands 27/1(2011-03-01), 25-35 0393-5965 27:1<25 2011 27 10453 https://doi.org/10.1007/s10453-010-9173-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10453-010-9173-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Griffin Dale US Geological Survey, Geologic Discipline, 2639 North Monroe Street, Suite A200, 32303, Tallahassee, FL, USA aut NATIONALLICENCE 700 1- Gonzalez Cristina University of La Laguna, University Institute of Tropical Diseases and Public Health, Avda. Francisco Sanchez, 38271, La Laguna, Tenerife, Canary Islands, Spain aut NATIONALLICENCE 700 1- Teigell Nuria University of La Laguna, University Institute of Tropical Diseases and Public Health, Avda. Francisco Sanchez, 38271, La Laguna, Tenerife, Canary Islands, Spain aut NATIONALLICENCE 700 1- Petrosky Terry US Geological Survey, Water Resources Discipline, 2639 North Monroe Street, Suite A200, 32303, Tallahassee, FL, USA aut NATIONALLICENCE 700 1- Northup Diana University of New Mexico, Biology, MSC03 2020, 1 University of New Mexico, 87131, Albuquerque, NM, USA aut NATIONALLICENCE 700 1- Lyles Mark US Navy, Research Program Integration and Mission Development, Bureau of Medicine and Surgery, 2300 East Street, NW, Bldg 5, 20372-5300, Washington, DC, USA aut NATIONALLICENCE 773 0- Aerobiologia Springer Netherlands 27/1(2011-03-01), 25-35 0393-5965 27:1<25 2011 27 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer