caa a22 4500 445863935 CHVBK 20180317145452.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10453-010-9184-9 doi (NATIONALLICENCE)springer-10.1007/s10453-010-9184-9 Airborne Legionella bacteria from pulp waste treatment plant: aerosol particles characterized as aggregates and their potential hazard [Elektronische Daten] [Janet Blatny, Jim Ho, Gunnar Skogan, Else Fykse, Tone Aarskaug, Viggo Waagen] In response to reported outbreaks of Legionnaires' disease near a biological waste treatment plant, Legionella-containing aerosols were characterized at this location. Culturable bacteria-containing particles were assessed with slit-to-agar samplers and liquid impingement. Results showed that the air at this location contained a higher level of airborne Legionella particles compared to that of a control clean location 90km away. On average, about 1 of 50 culturable particles contained Legionella (2%). The median particle size was estimated to 3.5μm permitting an estimation of a maximum about 147 Legionella cells per aggregate. This implies that in <11h, a human under such conditions (worse-case), may inhale an estimated lethal dose of individual Legionella cells (1,000 cells, 100% alveolar retained deposition level). Additionally, 44 taxonomically different airborne bacterial genera were measured at the treatment plant using denaturing gradient-gel electrophoresis (DGGE) and 16S rDNA analysis of air samples. Of these, 64% of the genera were not detected at the clean location. Our findings suggest that the treatment facility may emit respirable particles, as complex aggregates, containing a mixture of several Legionella cells and other bacterial cells. These findings can potentially have an impact on assessing environmental risk exposure and health hazard prediction modeling. Springer Science+Business Media B.V., 2010 Legionella nationallicence Aerosol particles nationallicence Aggregates nationallicence Environmental hazard nationallicence Waste treatment facility nationallicence Blatny Janet Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut Ho Jim Defence Research and Development Canada (DRDC), Suffield, P. O. Box 4000, T1A 8K6, Medicine Hat, Alberta, Canada aut Skogan Gunnar Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut Fykse Else Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut Aarskaug Tone Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut Waagen Viggo Borregaard, P. O. Box 162, N-1701, Sarpsborg, Norway aut Aerobiologia Springer Netherlands 27/2(2011-06-01), 147-162 0393-5965 27:2<147 2011 27 10453 https://doi.org/10.1007/s10453-010-9184-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10453-010-9184-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blatny Janet Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut NATIONALLICENCE 700 1- Ho Jim Defence Research and Development Canada (DRDC), Suffield, P. O. Box 4000, T1A 8K6, Medicine Hat, Alberta, Canada aut NATIONALLICENCE 700 1- Skogan Gunnar Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut NATIONALLICENCE 700 1- Fykse Else Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut NATIONALLICENCE 700 1- Aarskaug Tone Norwegian Defence Research Establishment (FFI), P. O. Box 25, N-2027, Kjeller, Norway aut NATIONALLICENCE 700 1- Waagen Viggo Borregaard, P. O. Box 162, N-1701, Sarpsborg, Norway aut NATIONALLICENCE 773 0- Aerobiologia Springer Netherlands 27/2(2011-06-01), 147-162 0393-5965 27:2<147 2011 27 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer