caa a22 4500 445863951 CHVBK 20180317145452.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10453-010-9185-8 doi (NATIONALLICENCE)springer-10.1007/s10453-010-9185-8 Feasibility of using real-time optical methods for detecting the presence of viable bacteria aerosols at low concentrations in clean room environments [Elektronische Daten] [Jim Ho, Nicholas Stanley, Thomas Kuehn] An experimental investigation was carried out to determine the agreement between two methods of viable bacteria aerosol detection. Various amounts of Bacillus globigii (BG) spores were aerosolized in 1-s bursts into a HEPA-filtered air stream and sampled simultaneously with a fluorescence aerosol particle sensor (FLAPS) and a slit to agar biological air sampler. The slit sampler incorporated 150-mm malt extract culture plates, which were incubated at 37°C for at least 12h before culturable BG particles were counted in terms of colony-forming units (CFU). A relationship between CFU and optically detected viable bacteria particles was determined as culturable particle concentrations decreased. Through further analytical procedures, the FLAPS showed a limit of detection (LOD) of 4.2 bacterial particle/2.5l of sampled air or 1.7×103m−3. This real-time bacteria aerosol monitor could be used to detect burst contamination events during a surgical procedure. The technology may be used for developing a dose-response relationship between bacterial particle exposure and infection, a tool potentially helpful in determining patient risk. Her Majesty the Queen in Rights of Canada, 2010 FLAPS nationallicence Fluorescent aerosol sensor nationallicence Hospital bacteria detection nationallicence Limit of detection nationallicence Operating room monitoring nationallicence Ho Jim Defence Research and Development Canada Suffield, Detection and Identification Section, Station Main, PO Box 4000, T1A 8K6, Medicine Hat, AB, Canada aut Stanley Nicholas Department of Mechanical Engineering, University of Minnesota, 55455, Minneapolis, MN, USA aut Kuehn Thomas Department of Mechanical Engineering, University of Minnesota, 55455, Minneapolis, MN, USA aut Aerobiologia Springer Netherlands 27/2(2011-06-01), 163-172 0393-5965 27:2<163 2011 27 10453 https://doi.org/10.1007/s10453-010-9185-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10453-010-9185-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ho Jim Defence Research and Development Canada Suffield, Detection and Identification Section, Station Main, PO Box 4000, T1A 8K6, Medicine Hat, AB, Canada aut NATIONALLICENCE 700 1- Stanley Nicholas Department of Mechanical Engineering, University of Minnesota, 55455, Minneapolis, MN, USA aut NATIONALLICENCE 700 1- Kuehn Thomas Department of Mechanical Engineering, University of Minnesota, 55455, Minneapolis, MN, USA aut NATIONALLICENCE 773 0- Aerobiologia Springer Netherlands 27/2(2011-06-01), 163-172 0393-5965 27:2<163 2011 27 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer