caa a22 4500 44586396X CHVBK 20180317145452.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10453-010-9181-z doi (NATIONALLICENCE)springer-10.1007/s10453-010-9181-z Diversity of airborne bacteria in samples collected using different devices for aerosol collection [Elektronische Daten] [Camilla Fahlgren, Gunnar Bratbak, Ruth-Anne Sandaa, Runar Thyrhaug, Ulla Zweifel] Bacteria are ubiquitous in the atmosphere, where they form a highly diverse community, albeit low in abundance. Several approaches are available for collecting airborne particles, though few comparative studies have been conducted to date. This study examined how different sampling strategies affect the apparent composition of the airborne community. Three devices were tested: an impactor, a liquid impinger, and a Teflon membrane filter. Comparative studies were conducted at one mountainous location in Norway and one seaside location in Sweden. At both locations, microbial samples were collected in parallel using the sampling devices. DNA extraction, construction of 16S rRNA gene clone libraries, and subsequent sequencing were used to identify the bacteria. The comparison between clone libraries retrieved using the different devices indicated good agreement regarding dominant species, overall diversity, and distribution of species among phylogenetic groups. Among the less common species, there were few shared sequences in different clone libraries, likely due to the high diversity of the assessed samples. Bacteria belonging to the Bacteroidetes and Proteobacteria phyla dominated at both locations, and the most common genera were Sphingomonas sp. and Pantoea sp. Chloroplast-like 16S rRNA gene sequences were detected in all samples. Springer Science+Business Media B.V., 2010 Airborne bacteria nationallicence Bacterial diversity nationallicence Aerosol collection nationallicence Aerobiology nationallicence Fahlgren Camilla School of Natural Sciences, Linnaeus University, SE-391 82, Kalmar, Sweden aut Bratbak Gunnar Department of Biology, University of Bergen, Pb 7803 N-5020, Bergen, Norway aut Sandaa Ruth-Anne Department of Biology, University of Bergen, Pb 7803 N-5020, Bergen, Norway aut Thyrhaug Runar Department of Biology, University of Bergen, Pb 7803 N-5020, Bergen, Norway aut Zweifel Ulla Department of Cell and Molecular Biology, University of Gothenburg, SE-405 30, Göteborg, Sweden aut Aerobiologia Springer Netherlands 27/2(2011-06-01), 107-120 0393-5965 27:2<107 2011 27 10453 https://doi.org/10.1007/s10453-010-9181-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10453-010-9181-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fahlgren Camilla School of Natural Sciences, Linnaeus University, SE-391 82, Kalmar, Sweden aut NATIONALLICENCE 700 1- Bratbak Gunnar Department of Biology, University of Bergen, Pb 7803 N-5020, Bergen, Norway aut NATIONALLICENCE 700 1- Sandaa Ruth-Anne Department of Biology, University of Bergen, Pb 7803 N-5020, Bergen, Norway aut NATIONALLICENCE 700 1- Thyrhaug Runar Department of Biology, University of Bergen, Pb 7803 N-5020, Bergen, Norway aut NATIONALLICENCE 700 1- Zweifel Ulla Department of Cell and Molecular Biology, University of Gothenburg, SE-405 30, Göteborg, Sweden aut NATIONALLICENCE 773 0- Aerobiologia Springer Netherlands 27/2(2011-06-01), 107-120 0393-5965 27:2<107 2011 27 10453 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer