caa a22 4500 445865857 CHVBK 20180317145457.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1140/epjd/e2011-10679-1 doi (NATIONALLICENCE)springer-10.1140/epjd/e2011-10679-1 Heavy ion induced damage to plasmid DNA: plateau region vs. spread out Bragg-peak [Elektronische Daten] [H. Dang, M. van Goethem, E. van der Graaf, S. Brandenburg, R. Hoekstra, T. Schlathölter] We have investigated the damage of synthetic plasmid pBR322 DNA in dilute aqueous solutions induced by fast carbon ions. The relative contribution of indirect damage and direct damage to the DNA itself is expected to vary with linear energy transfer along the ion track, with the direct damage contribution increasing towards the Bragg peak. Therefore, 12C ions at the spread-out Bragg peak (dose averaged LET∞ = 189 ± 15 keV/μm) and in the plateau region of the Bragg curve (LET = 40 keV/μm) were employed and the radical scavenger concentration in the plasmid solution was varied to quantify the indirect effect. In order to minimize the influence of 12C break-up fragments, a relatively low initial energy of 90 MeV/nucleon was employed for the carbon ions. DNA damage has been quantified by subsequent electrophoresis on agarose gels. We find that strand breaks due to both indirect and direct effects are systematically higher in the plateau region as compared to the Bragg peak region with the difference being smallest at high scavenging capacities. In view of the fact that the relative biological effectiveness for many biological endpoints is maximum at the Bragg peak our findings imply that DNA damage at the Bragg peak is qualitatively most severe. EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg, 2011 Dang H. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut van Goethem M. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut van der Graaf E. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut Brandenburg S. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut Hoekstra R. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut Schlathölter T. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut The European Physical Journal D Springer-Verlag 63/3(2011-08-01), 359-367 1434-6060 63:3<359 2011 63 10053 https://doi.org/10.1140/epjd/e2011-10679-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1140/epjd/e2011-10679-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dang H. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut NATIONALLICENCE 700 1- van Goethem M. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut NATIONALLICENCE 700 1- van der Graaf E. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Brandenburg S. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Hoekstra R. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut NATIONALLICENCE 700 1- Schlathölter T. KVI University of Groningen, Zernikelaan 25, 9747AA, Groningen, The Netherlands aut NATIONALLICENCE 773 0- The European Physical Journal D Springer-Verlag 63/3(2011-08-01), 359-367 1434-6060 63:3<359 2011 63 10053 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer