caa a22 4500 445869844 CHVBK 20180317145508.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11101-011-9210-8 doi (NATIONALLICENCE)springer-10.1007/s11101-011-9210-8 Engineering secondary metabolite production in hairy roots [Elektronische Daten] [Sheela Chandra, Ramesh Chandra] Plants are a rich source of valuable secondary metabolites. Plant cell culture technologies developed in the past as possible tools for secondary metabolites production. In most cases, for the large scale production of these compounds, production is too low for commercialization. The cultured cell suspensions being undifferentiated have not gained momentum because of their instability and non-uniformity of the product formation. Secondary metabolite production is under strict metabolic regulation and tissue specific localization. Hence the differentiated cultures such as hairy root cultures are widely studied. Agrobacterium rhizogenes causes hairy root disease in plants. Transformed roots produced by A. rhizogenes infection are characterized by high growth rate, genetic stability and growth in hormone free media. These genetically transformed root cultures can produce amounts of secondary metabolites comparable to that of intact plants. Elicitation of hairy roots leads to increased production of secondary metabolites and also helps in designing of metabolic traps to allow adsorption of product, preventing feedback inhibition and protection of metabolites from degradation in the culture media. Permeabilization and in situ product adsorption result in many fold increase in product yield. T- DNA activation tagging allows overexpressing the respective gene and increasing the product formation in transformed hairy roots. Recent progress in transgenic research has opened up the possibility of the metabolic engineering of biosynthetic pathways to produce high-value secondary metabolites. Metabolic engineering offers promising perspectives to improve yields; however it requires the understanding of the regulation of the secondary metabolite pathways involved in the regulation of levels of product, enzymes and genes, including aspects as transport and compartmentation. This article reviews the recent advances in secondary metabolites production in transformed hairy roots. Springer Science+Business Media B.V., 2011 Elicitation nationallicence Hairy roots nationallicence Metabolic engineering nationallicence Secondary metabolites nationallicence Transcriptional factors nationallicence DXR : 1-Deoxy-D-xylulose 5-phosphate reductoisomerase nationallicence H6H : Hyoscyamine 6 β hydroxylase nationallicence PMT : Putrescine N-methyltransferase nationallicence PAL : Phenylalanine ammonia-lyase nationallicence PEG8000 : Polyethylene glycol, molecular weight 8000 nationallicence Rubisco : Ribulose bisphosphate carboxylase nationallicence STR : Strictosidine synthase nationallicence TDC : Tryptophan decarboxylase nationallicence Chandra Sheela Department of Biotechnology, Birla Institute of Technology, Mesra, 835215, Ranchi, Jharkhand, India aut Chandra Ramesh Department of Biotechnology, Birla Institute of Technology, Mesra, 835215, Ranchi, Jharkhand, India aut Phytochemistry Reviews Springer Netherlands 10/3(2011-09-01), 371-395 1568-7767 10:3<371 2011 10 11101 https://doi.org/10.1007/s11101-011-9210-8 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11101-011-9210-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chandra Sheela Department of Biotechnology, Birla Institute of Technology, Mesra, 835215, Ranchi, Jharkhand, India aut NATIONALLICENCE 700 1- Chandra Ramesh Department of Biotechnology, Birla Institute of Technology, Mesra, 835215, Ranchi, Jharkhand, India aut NATIONALLICENCE 773 0- Phytochemistry Reviews Springer Netherlands 10/3(2011-09-01), 371-395 1568-7767 10:3<371 2011 10 11101 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer