caa a22 4500 445870044 CHVBK 20180317145508.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s11101-010-9179-8 doi (NATIONALLICENCE)springer-10.1007/s11101-010-9179-8 Blockage of chloride channels and anion transporters with pesticidal natural products and their synthetic analogs [Elektronische Daten] [Dhana Boina, Jeffrey Bloomquist] Ligand-gated chloride channels mediate a variety of functions in excitable membranes of nerve and muscle in insects, and have a long history as targets for neurotoxic insecticides. Recent findings from our laboratory confirm that the natural product silphinenes and their semi-synthetic analogs share a mode of action with the established ligand-gated chloride channel antagonist, picrotoxinin. The silphinenes are non-selective, being roughly equipotent on insect and mammalian receptors, but also possess lethal and neurotoxic effects on a dieldrin-resistant strain of Drosophila melanogaster. These findings suggest that silphinenes act on insect GABA receptors in a way that is different from picrotoxinin, and it is possible that resistant insect populations in the field could be controlled with insecticidal compounds derived from the silphinenes. Voltage-gated chloride channels and anion transporters provide additional classes of validated targets for insecticidal/nematicidal action. Anion transporter blockers are toxic to insects via an action on the gut, and RNAi studies implicate voltage-gated chloride channels in nematode muscle as another possible target. There was no cross resistance to DIDS in a dieldrin-resistant strain of Drosophila melanogaster, and no evidence for neurotoxicity. The potent paralytic actions of anion transporter blockers against nematodes, and stomach poisoning activity against lepidopteran larvae suggests they are worthy of further investigation as commercial insecticidal/nematicidal agents. Springer Science+Business Media B.V., 2010 Silphinene nationallicence GABA receptor nationallicence Fipronil nationallicence Fipronil analog nationallicence CLC nationallicence rdl nationallicence Insecticide resistance nationallicence GluRCl nationallicence Glutamate receptor nationallicence Ligand-gated chloride channel nationallicence Voltage-sensitive chloride channel nationallicence Anion transporter nationallicence ATs : Anion transporters nationallicence 9-AC : Anthracene-9-carboxylic acid nationallicence CNS : Central nervous system nationallicence CBEs : Chloride-bicarbonate exchangers nationallicence DIDS : 4,4′-diisothiocyanatostilbene 2,2′-disulfonic acid nationallicence DST : 3,5-dihydroxy-4-isopropyl stilbene nationallicence GluRCl : Glutamate-gated chloride channels nationallicence IAA-94 : Indanyloxy acetic acid nationallicence LGCCs : Ligand-gated chloride channels nationallicence NPPB : 5-nitro-2-(3-phenylpropylamino) benzoic acid nationallicence rdl : Resistance to dieldrin nationallicence VGCCs : Voltage-gated chloride channels nationallicence Boina Dhana Department of Entomology and Nematology, Citrus Research and Education Center, University of Florida, 33850, Lake Alfred, FL, USA aut Bloomquist Jeffrey Department of Entomology and Nematology, Emerging Pathogens Institute, University of Florida, 2055 Mowry Road, PO Box 100009, 32610-00009, Gainesville, FL, USA aut Phytochemistry Reviews Springer Netherlands 10/2(2011-06-01), 217-226 1568-7767 10:2<217 2011 10 11101 https://doi.org/10.1007/s11101-010-9179-8 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11101-010-9179-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boina Dhana Department of Entomology and Nematology, Citrus Research and Education Center, University of Florida, 33850, Lake Alfred, FL, USA aut NATIONALLICENCE 700 1- Bloomquist Jeffrey Department of Entomology and Nematology, Emerging Pathogens Institute, University of Florida, 2055 Mowry Road, PO Box 100009, 32610-00009, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Phytochemistry Reviews Springer Netherlands 10/2(2011-06-01), 217-226 1568-7767 10:2<217 2011 10 11101 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer