caa a22 4500 445878509 CHVBK 20180317145535.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s11030-010-9290-1 doi (NATIONALLICENCE)springer-10.1007/s11030-010-9290-1 Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues [Elektronische Daten] [Montserrat Terrazas, Ramon Eritja] Oligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2′-OH position proceeded in good yield and very high 2′-regioselectivity. RNA duplexes carrying 5-ethyluridine either at the sense or the guide strands display RNAi activity comparable to or slightly better than that of unmodified RNA duplexes. Gene suppression experiments using luciferase targets in SH-SY5Y cells show that the ethyl group is generally well accepted at all positions although a small decrease in RNA interference activity is observed when one 5-ethylU residue is incorporated in the 3′ overhangs. Springer Science+Business Media B.V., 2010 SiRNA nationallicence RNA interference nationallicence 5-Ethyluridine nationallicence Dual luciferase assay nationallicence Oligonucleotide synthesis nationallicence Solid-phase nationallicence as : Guide (antisense) strand nationallicence ACN : Acetonitrile nationallicence DIPEA : N,N-diisopropylethylamine nationallicence DMAP : N,N-dimethylaminopyridine nationallicence DMEM : Dulbecco's modified Eagle medium nationallicence DMT : 4,4′-Dimethoxytrityl nationallicence EtOAc : Ethyl acetate nationallicence FBS : Fetal bovine serum nationallicence HEPES : 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid nationallicence MeOH : Methanol nationallicence siRNA : Small interfering RNA nationallicence ss : Sense strand nationallicence TBAF : Tetrabutylammonium fluoride nationallicence TBDMS : tert-Butyldimethylsilyl nationallicence TEAA : Triethylammonium acetate nationallicence Terrazas Montserrat Institute for Research in Biomedicine (IRB Barcelona), Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Baldiri i Reixac 10, 08028, Barcelona, Spain aut Eritja Ramon Institute for Research in Biomedicine (IRB Barcelona), Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Baldiri i Reixac 10, 08028, Barcelona, Spain aut Molecular Diversity Springer Netherlands 15/3(2011-08-01), 677-686 1381-1991 15:3<677 2011 15 11030 https://doi.org/10.1007/s11030-010-9290-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11030-010-9290-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Terrazas Montserrat Institute for Research in Biomedicine (IRB Barcelona), Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Baldiri i Reixac 10, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Eritja Ramon Institute for Research in Biomedicine (IRB Barcelona), Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Baldiri i Reixac 10, 08028, Barcelona, Spain aut NATIONALLICENCE 773 0- Molecular Diversity Springer Netherlands 15/3(2011-08-01), 677-686 1381-1991 15:3<677 2011 15 11030 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer