caa a22 4500 445878967 CHVBK 20190327083712.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s11030-010-9297-7 doi (NATIONALLICENCE)springer-10.1007/s11030-010-9297-7 HPLC-based activity profiling for GABAA receptor modulators from the traditional Chinese herbal drug Kushen ( Sophora flavescens root) [Elektronische Daten] [Xinzhou Yang, Igor Baburin, Inken Plitzko, Steffen Hering, Matthias Hamburger] An EtOAc extract from the roots of Sophora flavescens (Kushen) potentiated γ-aminobutyric acid (GABA)-induced chloride influx in Xenopus oocytes transiently expressing GABAA receptors with subunit composition, α 1 β 2 γ 2S. HPLC-based activity profiling of the extract led to the identification of 8-lavandulyl flavonoids, kushenol I, sophoraflavanone G, (−)-kurarinone, and kuraridine as GABAA receptor modulators. In addition, a series of inactive structurally related flavonoids were characterized. Among these, kushenol Y (4) was identified as a new natural product. The 8-lavandulyl flavonoids are first representatives of a novel scaffold for the target. Springer Science+Business Media B.V., 2011 GABAA receptor modulators nationallicence HPLC-based activity profiling nationallicence 8-Lavandulyl flavonoids nationallicence Sophora flavescens nationallicence Fabaceae nationallicence TOFMS : Time of flight mass spectrometry nationallicence PDA : Photodiode array nationallicence HMBC : Heteronuclear multiple-bond correlation nationallicence HSQC : Heteronuclear single quantum coherence nationallicence CD : Circular dichroism nationallicence Yang Xinzhou Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland aut Baburin Igor Institute of Pharmacology and Toxicology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Plitzko Inken Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland aut Hering Steffen Institute of Pharmacology and Toxicology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut Hamburger Matthias Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland aut Molecular Diversity Springer Netherlands 15/2(2011-05-01), 361-372 1381-1991 15:2<361 2011 15 11030 https://doi.org/10.1007/s11030-010-9297-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11030-010-9297-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xinzhou Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland aut NATIONALLICENCE 700 1- Baburin Igor Institute of Pharmacology and Toxicology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 700 1- Plitzko Inken Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland aut NATIONALLICENCE 700 1- Hering Steffen Institute of Pharmacology and Toxicology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria aut NATIONALLICENCE 700 1- Hamburger Matthias Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland aut NATIONALLICENCE 773 0- Molecular Diversity Springer Netherlands 15/2(2011-05-01), 361-372 1381-1991 15:2<361 2011 15 11030 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 445878967 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer