caa a22 4500 445879165 CHVBK 20180317145537.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s11030-010-9240-y doi (NATIONALLICENCE)springer-10.1007/s11030-010-9240-y Prediction of mucin-type O -glycosylation sites by a two-staged strategy [Elektronische Daten] [YuDong Cai, JianFeng He, Lin Lu] The mucin-type O-glycosylation of a protein is an important type of protein post-translational modification. This process is mediated by a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases which transfer the N-acetylgalactosamine (GalNAc) to the serine or threonine residues with unknown specificity. In order to determine the glycosylation sites of a given protein, we present a two-staged prediction method here, which first determines whether a protein is a glycoprotein, and then determines the glycosylation sites of a protein that has been predicted to be glycosylated in the first stage. In the first stage, a protein is encoded by the protein families in PFAM, which is a collective annotated database of classified protein families; then it is predicted by a predictor trained by the training set. In the second stage, nonapeptides of the predicted mucin-type glycoproteins, with serine or threonine residues at their fifth sites, are represented by indices in AAIndex. Then, it is predicted whether the nonapeptides are attached by GalNAc by a predictor, which is constructed with features selected by feature selection methods [Maximum Relevance Minimum Redundancy (mRMR) method and Incremental Feature Selection method]. The prediction accuracy of the first stage is 94.9% validated by Leave-One-Out validation method; the prediction accuracy of the second stage is 99.4%. These results show that this method is valuable to study the mucin-type O-glycosylation. The analysis of the features used to construct the predictor of the second stage confirms the previously obtained results from other groups. The residues at position −1 and +3 have great impact on the prediction. Among other amino acid indices, the indices about alpha and turn propensities and indices about hydrophobicity of the residues in nonapeptide also influence the recognition of the GalNAc transferases. A web server is available at http://chemdata.shu.edu.cn/gal/. Springer Science+Business Media B.V., 2010 Mucin-type O -glycosylation sites nationallicence PFAM nationallicence Minimum Redundancy Maximum Relevance nationallicence Feature Selection nationallicence Nearest Neighbor Algorithm nationallicence K-fold cross-validation nationallicence mRMR : The Maximum Relevance Minimum Redundancy nationallicence NNA : Nearest Neighbor Algorithm nationallicence AAIndex : Amino Acid Index nationallicence IFS method : Incremental Feature Selection nationallicence Cai YuDong Institute of System Biology, Shanghai University, 99 Shangda Road, 200244, Shanghai, People's Republic of China aut He JianFeng Department of Biomedical Engineering, Shanghai Jiao Tong University, 200040, Shanghai, People's Republic of China aut Lu Lin Department of Biomedical Engineering, Shanghai Jiao Tong University, 200040, Shanghai, People's Republic of China aut Molecular Diversity Springer Netherlands 15/2(2011-05-01), 427-433 1381-1991 15:2<427 2011 15 11030 https://doi.org/10.1007/s11030-010-9240-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11030-010-9240-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai YuDong Institute of System Biology, Shanghai University, 99 Shangda Road, 200244, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- He JianFeng Department of Biomedical Engineering, Shanghai Jiao Tong University, 200040, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Lu Lin Department of Biomedical Engineering, Shanghai Jiao Tong University, 200040, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Molecular Diversity Springer Netherlands 15/2(2011-05-01), 427-433 1381-1991 15:2<427 2011 15 11030 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer