caa a22 4500 445879378 CHVBK 20180317145537.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0504-0 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0504-0 Simplified determination of bacterial contamination by Escherichia coli using a flow injection system with piezoelectric detection [Elektronische Daten] [María Plata, Ana Contento, Ángel Ríos] A rapid and automated method was developed for the determination of bacterial contamination and using Escherichia coli as a model microorganism. The method involves the use of a sensor connected to a flow injection (FI) system. The sample is introduced through a flow injection system into a piezoelectric quartz crystal (PQC) flow-cell. The resulting change of the resonance frequency is related to the bacterial contamination in the sample. The parameters associated with the flow system and the conditions for introducing the sample culture were optimized. Calibration curves are linear in the range from 3.2 × 107 to 3.2 × 109cfu per mL-1, with a correlation coefficient of 0.997. The reproducibility was between 3.1 and 7.6%, and the detection limit is 1.1 × 107cfu per mL-1. The method allowed the determination of bacterial contamination in residual water and in samples of milk and chicken stock within 5h, while the conventional plate count method requires 24 to 48h. The results obtained by these two methods are in good agreement. Figure A rapid and automated method for the determination of bacterial growth contamination is proposed and Escherichia coli was used as a model microorganism. The methodology involves the use of a piezoelectric quartz crystal (PQC) sensor connected to a flow injection (FI) system. The sample was introduced through the FI system and the resonant frequency change of PQC is related to the bacterial contamination in the sample. The method allows the successful determination of bacteria in residual water and several food samples Springer-Verlag, 2010 Piezoelectric sensor nationallicence Flow system nationallicence Bacterial contamination nationallicence Escherichia coli nationallicence Food and water samples nationallicence Plata María Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Avda. Camilo José Cela s/n, E-13071, Ciudad Real, Spain aut Contento Ana Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Avda. Camilo José Cela s/n, E-13071, Ciudad Real, Spain aut Ríos Ángel Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Avda. Camilo José Cela s/n, E-13071, Ciudad Real, Spain aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 447-454 0026-3672 172:3-4<447 2011 172 604 https://doi.org/10.1007/s00604-010-0504-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0504-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Plata María Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Avda. Camilo José Cela s/n, E-13071, Ciudad Real, Spain aut NATIONALLICENCE 700 1- Contento Ana Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Avda. Camilo José Cela s/n, E-13071, Ciudad Real, Spain aut NATIONALLICENCE 700 1- Ríos Ángel Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, Avda. Camilo José Cela s/n, E-13071, Ciudad Real, Spain aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 447-454 0026-3672 172:3-4<447 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer