caa a22 4500 445879416 CHVBK 20180317145537.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0495-x doi (NATIONALLICENCE)springer-10.1007/s00604-010-0495-x Highly sensitive deoxynivalenol immunosensor based on a glassy carbon electrode modified with a fullerene/ferrocene/ionic liquid composite [Elektronische Daten] [Wei Zhilei, Sun Xiulan, Li Zaijun, Fang Yinjun, Ren Guoxiao, Huang Yaru, Liu Junkang] We describe a sensitive electrochemical immunosensor for the detection of deoxynivalenol (DON). It is based on a glassy carbon electrode modified with a composite made from fullerene (C60), ferrocene and the ionic liquid. The components were immobilized on the surface of the electrode using chitosan cross-linked with epichlorohydrin. Then, the antibody to DON was covalently conjugated to the surface which then was blocked with serum albumin. The performance of the immunosensor was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. It offers good repeatability (RSD = 1.2%), selectivity, a stability of more than 180days, an impedimetric response to DON in the range of 1pgmL−1 to 0.3ng mL−1, and a detection limit (at S/N = 3) of 0.3pgmL−1. The limit of detection is better than that of GC, HPLC, GC-MS, HPLC-MS and LC-MS-MS. The effects of omitting C60 or the ionic liquid were also examined. The results indicate that the sensitivity of the biosensor is 2-fold better if C60 and ionic liquids are used. This demonstrates that C60 facilitates electron transfer on the surface of the modified electrode due to its unique electrochemical properties, while the ionic liquid provides a biocompatible microenvironment for the antibody. This results in increased sensitivity and stability. The method was satisfactorily applied to the determination of DON in food samples. Figure Fullerene, ferrocene, chitosan and ionic liquid offer remarkable synergistic contributions towards improve electrochemical performance of DON sensor. This results that novel sensor exhibits a good repeatability (RSD=1.2%), selectivity, very low detection limit (S/N=3) of 0.0003 ng mL-1, an impedimetric response to DON in the range from 0.001 ng mL-1 to 0.3 ng mL-1 and a stability of more than 180 days. Cyclic voltammograms of, Ab/C60-FC-IL-GCE a and Ab/FC-IL-GCE b Springer-Verlag, 2010 Fullerene nationallicence Ferrocene nationallicence Ionic liquid nationallicence Deoxynivalenol nationallicence Immunosensor nationallicence Zhilei Wei School of Chemical and Materials Engineering, Jiangnan University, 214122, Wuxi, China aut Xiulan Sun State Key Laboratory of Food Science and Technology, 214122, Wuxi, China aut Zaijun Li School of Chemical and Materials Engineering, Jiangnan University, 214122, Wuxi, China aut Yinjun Fang Zhejiang Zanyu Technology Limited Corporation, 311215, Hangzhou, China aut Guoxiao Ren Zhejiang Zanyu Technology Limited Corporation, 311215, Hangzhou, China aut Yaru Huang Zhejiang Zanyu Technology Limited Corporation, 311215, Hangzhou, China aut Junkang Liu School of Chemical and Materials Engineering, Jiangnan University, 214122, Wuxi, China aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 365-371 0026-3672 172:3-4<365 2011 172 604 https://doi.org/10.1007/s00604-010-0495-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0495-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhilei Wei School of Chemical and Materials Engineering, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Xiulan Sun State Key Laboratory of Food Science and Technology, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Zaijun Li School of Chemical and Materials Engineering, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Yinjun Fang Zhejiang Zanyu Technology Limited Corporation, 311215, Hangzhou, China aut NATIONALLICENCE 700 1- Guoxiao Ren Zhejiang Zanyu Technology Limited Corporation, 311215, Hangzhou, China aut NATIONALLICENCE 700 1- Yaru Huang Zhejiang Zanyu Technology Limited Corporation, 311215, Hangzhou, China aut NATIONALLICENCE 700 1- Junkang Liu School of Chemical and Materials Engineering, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 365-371 0026-3672 172:3-4<365 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer