caa a22 4500 445879459 CHVBK 20180317145537.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0519-6 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0519-6 DNA biosensors based on metallo-intercalator probes and electrocatalytic amplification [Elektronische Daten] [Ming-Yuan Wei, Liang-Hong Guo, Parviz Famouri] Strategies for electrochemical sensing of DNA can be classified into label-free and label-based approaches, categories of which include enzyme-, nanomaterial- and redox labels that are attached to DNA either by covalent or non-covalent means. Metallointercalators represent one group of small molecule redox labels that non-covalently enter the groove of a DNA. The metallointercalator plays a dual-role in acting as a structure indicator (for hybridization) and a signal generator. Labeling is not needed, and electrochemical measurements can be carried out in a label-free solution of an electrolyte. However, such metallointercalators lack the option of catalytic signal generation as in the case of enzyme- and nanomaterial-based labels. Therefore, signal amplification becomes crucial. We first survey here recent progress in this area. A signal-amplifying system is presented that relies on the electroatalytic oxidation of a metallointercalator ruthenium(II)bipyridine/phenoxazine complex in the presence of electron donor species such as oxalate, DNA bases, or tripropylamine. Recent work on such DNA sensors is discussed. Results suggest that such metallointercalator-based DNA sensors represent a viable platform for developing high-throughput and automated PCR/lab-on-a-chip devices as well as visualized multifunctional DNA sensors. DNA biosensors based on metallo-intercalator probes and electrocatalytic amplification Springer-Verlag, 2010 Metallointercalator nationallicence Biosensor nationallicence Signal amplification nationallicence DNA binding nationallicence DNA damage nationallicence Wei Ming-Yuan Lane Department of Computer Science and Electrical Engineering, College of Engineering and Mineral Resources, West Virginia University, 26506-6109, Morgantown, WV, USA aut Guo Liang-Hong State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut Famouri Parviz Lane Department of Computer Science and Electrical Engineering, College of Engineering and Mineral Resources, West Virginia University, 26506-6109, Morgantown, WV, USA aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 247-260 0026-3672 172:3-4<247 2011 172 604 https://doi.org/10.1007/s00604-010-0519-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0519-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Ming-Yuan Lane Department of Computer Science and Electrical Engineering, College of Engineering and Mineral Resources, West Virginia University, 26506-6109, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Guo Liang-Hong State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China aut NATIONALLICENCE 700 1- Famouri Parviz Lane Department of Computer Science and Electrical Engineering, College of Engineering and Mineral Resources, West Virginia University, 26506-6109, Morgantown, WV, USA aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 247-260 0026-3672 172:3-4<247 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer