caa a22 4500 44587953X CHVBK 20180317145538.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0498-7 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0498-7 Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on a gold electrode modified with a composite membrane made from an ionic liquid, ZnO nanoparticles and chitosan, and by using acridine orange as a redox indicator [Elektronische Daten] [Shafiquzzaman Siddiquee, Nor Yusof, Abu Salleh, Soon Tan, Fatimah Bakar] An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10-14 to 1.8 × 10-4molL-1, with a detection limit of 1.0 × 10-15molL-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms. Figure Suggested interaction mechanism of modified electrode (IL/ZnO/CHIT/AuE) between immobilization and hybridization Springer-Verlag, 2010 DNA electrochemical biosensor nationallicence DNA hybridization nationallicence ZnO nanopaticles nationallicence Ionic liquid nationallicence Chitosan nationallicence Trichoderma harzianum nationallicence Siddiquee Shafiquzzaman Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut Yusof Nor Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut Salleh Abu Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut Tan Soon Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut Bakar Fatimah Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 357-363 0026-3672 172:3-4<357 2011 172 604 https://doi.org/10.1007/s00604-010-0498-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0498-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siddiquee Shafiquzzaman Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Yusof Nor Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Salleh Abu Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Tan Soon Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Bakar Fatimah Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 357-363 0026-3672 172:3-4<357 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer