caa a22 4500 445879572 CHVBK 20180317145538.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0490-2 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0490-2 Ultratrace voltammetric method for the detection of DNA sequence related to human immunodeficiency virus type 1 [Elektronische Daten] [Ruibing Wang, Chenghu Xue, Mei Gao, Honglan Qi, Chengxiao Zhang] An ultra-trace voltammetric method was developed for the determination of single strand DNA (ss-DNA) related to the human immunodeficiency virus type 1 (HIV-1). It is based on the signal amplification of carbon nanotubes loaded with silver nanoparticles and placed on a gold microelectrode. The capture ss-DNA (a 21-mer) possessing a thiol group at the 3′ end was self-assembled onto the surface of the gold microelectrode. It was then hybridized with target HIV-1 ss-DNA (a 42-mer) and further hybridized with the electrochemical probe (a 18-mer ss-DNA) tagged with multiwall carbon nanotubes and loaded with silver nanoparticles. The resulting formation of a DNA sandwich conjugate led to a strong electrochemical oxidation signal that was linearly proportional to the concentration of HIV-1 ss-DNA in the range from 1.0 to 100pM. The detection limit was 0.5pM (at an S/N of 3). This was equivalent to 0.05fmol of HIV-1 ss-DNA in a volume of 20μL. The relative standard deviation was 4.0% at 1.0pM (n = 11). Non-complementary ss-DNA of HIV-1 ss-DNA was effectively discriminated. This work demonstrates that the employment of the microelectrode and a sandwich hybridization model is promising in terms of sensitive and selective electrochemical detection of DNA. Figure Schematic diagram of the sandwich electrochemical detection for DNA hybridization Springer-Verlag, 2010 DNA hybridization nationallicence Human immunodeficiency virus nationallicence Microelectrode nationallicence Multiwall carbon-nanotubes nationallicence Silver nanoparticle nationallicence Wang Ruibing School of Chemistry and Chemical Engineering, Yulin University, 719000, YuLin, People's Republic of China aut Xue Chenghu School of Chemistry and Chemical Engineering, Yulin University, 719000, YuLin, People's Republic of China aut Gao Mei Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, People's Republic of China aut Qi Honglan Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, People's Republic of China aut Zhang Chengxiao Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, People's Republic of China aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 291-297 0026-3672 172:3-4<291 2011 172 604 https://doi.org/10.1007/s00604-010-0490-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0490-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Ruibing School of Chemistry and Chemical Engineering, Yulin University, 719000, YuLin, People's Republic of China aut NATIONALLICENCE 700 1- Xue Chenghu School of Chemistry and Chemical Engineering, Yulin University, 719000, YuLin, People's Republic of China aut NATIONALLICENCE 700 1- Gao Mei Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Qi Honglan Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chengxiao Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, 710062, Xi'an, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 291-297 0026-3672 172:3-4<291 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer