caa a22 4500 445879599 CHVBK 20180317145538.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0513-z doi (NATIONALLICENCE)springer-10.1007/s00604-010-0513-z HPLC determination of perfluorinated carboxylic acids with fluorescence detection [Elektronische Daten] [Ewa Poboży, Edyta Król, Lena Wójcik, Mariusz Wachowicz, Marek Trojanowicz] Perfluorinated carboxylic acids (PFCAs) represent an important group of persistent perfluorinated organic compounds commonly determined in environmental and biological samples. A reversed-phase HPLC method was developed based on derivatization of the PFCAs with the commercially available fluorescent reagent 3-bromoacetyl coumarin. The method was optimized and this resulted in the efficient separation of PFCAs containing from 3 to 12 carbon atoms in molecule in 25min run. To improve sensitivity, the preconcentration step has been optimized using Oasis-WAX and C18 sorbents for SPE. A 100-fold preconcentration is achieved by solid-phase extraction with the sorbent C18 Sep-PAK to result in limits of detection in the range from 43 to 75ppt for the analytes examined, and in the application of the method of water analysis. Figure Chromatogram of mixture of perfluorinated carboxylic acids C3-PFCA - C12-PFCA with fluorescence detection after derivatization with 3-bromoacetyl coumarin (b), and blank (a) The Author(s), 2010 Perfluorinated carboxylic acids nationallicence HPLC nationallicence Fluorescence detection nationallicence Poboży Ewa Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut Król Edyta Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut Wójcik Lena Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut Wachowicz Mariusz Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut Trojanowicz Marek Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 409-417 0026-3672 172:3-4<409 2011 172 604 https://doi.org/10.1007/s00604-010-0513-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0513-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poboży Ewa Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Król Edyta Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Wójcik Lena Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Wachowicz Mariusz Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Trojanowicz Marek Department of Chemistry, University of Warsaw, Pasteura 1, PL 02-093, Warsaw, Poland aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 409-417 0026-3672 172:3-4<409 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer