caa a22 4500 445879610 CHVBK 20180317145538.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0502-2 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0502-2 Double layer enzyme modified carbon nanotubes as label for sandwich-type immunoassay of tumor markers [Elektronische Daten] [Min Liang, Ruo Yuan, Yaqin Chai, Ligen Min, Zhongju Song] An immunosensor was prepared for the determination of carcinoembryonic antigen (CEA). It is based on the use of multiwalled carbon nanotubes (MWCNTs) along with horseradish peroxidase-labeled antibody. The enzyme was assembled onto MWCNTs templates using the layer-by-layer technique and then conjugated to carcinoembryonic secondary antibodies (Ab2) as the enzyme label. The resulting assembly results in a largely amplified sensitivity. The response is linear in the range of 0.05 to 45ng mL-1, with a detection limit of 16.0pg mL-1. The immunosensor possesses good stability and good reproducibility. Figure A new immunosensor with double-layer enzyme-modified carbon nanotubes as label for sandwich-type tumor markers was proposed in this study Springer-Verlag, 2010 Double layer enzyme nationallicence Multi-walled carbon nanotubes nationallicence Immunosensor nationallicence Catalytic nationallicence Liang Min College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut Yuan Ruo College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut Chai Yaqin College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut Min Ligen College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut Song Zhongju College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 373-378 0026-3672 172:3-4<373 2011 172 604 https://doi.org/10.1007/s00604-010-0502-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0502-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liang Min College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Ruo College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Chai Yaqin College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Min Ligen College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Song Zhongju College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 373-378 0026-3672 172:3-4<373 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer