caa a22 4500 445879629 CHVBK 20180317145538.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00604-010-0487-x doi (NATIONALLICENCE)springer-10.1007/s00604-010-0487-x Electrochemiluminescence immunoassay at a nanoporous gold leaf electrode and using CdTe quantun dots as labels [Elektronische Daten] [Xiaoyu Li, Rongyue Wang, Xiaoli Zhang] An electrochemiluminescence-based immunoassay using quantum dots (QDs) as labels for the carcinoembryonic antigen (CEA) was developed using an electrode modified with leafs of nanoporous gold. CEA was initially immobilized on the electrode via a sandwich immunoreaction, and then CdTe quantum dots capped with thioglycolic acid were used to label the second antibody. The intensity of the ECL of the QDs reflects the quantity of CEA immobilized on the electrode. Thus, in the presence of dithiopersulfate as the coreactant, the ECL serves as the signal for the determination of CEA. The intensity of the electroluminescence (ECL) of the electrode was about 5.5-fold higher than that obtained with a bare gold electrode. The relation between ECL intensity and CEA concentration is linear in the range from 0.05 to 200ng.mL-1, and the detection limit is 0.01ng.mL-1. The method has the advantages of high sensitivity, good reproducibility and long-term stability, and paves a new avenue for applying quantum dots in ECL-based bioassays. Figure Electrochemiluminescence Immunoassay Based on CdTe Quantun Dots as labels at Nanoporous Gold Leaf electrode Springer-Verlag, 2010 Electrochemiluminescence nationallicence Immunoassay nationallicence Quantum dots nationallicence Nanoporous gold leaf electrode nationallicence Carcinoembryonic antigen nationallicence Li Xiaoyu School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China aut Wang Rongyue School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China aut Zhang Xiaoli School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China aut Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 285-290 0026-3672 172:3-4<285 2011 172 604 https://doi.org/10.1007/s00604-010-0487-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0487-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiaoyu School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Wang Rongyue School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 700 1- Zhang Xiaoli School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/3-4(2011-03-01), 285-290 0026-3672 172:3-4<285 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer