caa a22 4500 445879742 CHVBK 20180317145538.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00604-010-0468-0 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0468-0 Using ICP-OES and SEM-EDX in biosorption studies [Elektronische Daten] [Izabela Michalak, Katarzyna Chojnacka, Krzysztof Marycz] We have compared the analytical results obtained by inductively coupled plasma optical emission spectroscopy (ICP-OES) and by scanning electron microscopy with an energy dispersive X-ray analytical system (SEM-EDX) in order to explore the mechanism of metal ions biosorption by biomass using two independent methods. The marine macroalga Enteromorpha sp. was enriched with Cu(II), Mn(II), Zn(II), and Co(II) ions via biosorption, and the biosorption capacity of alga determined from the solution and biomass composition before and after biosorption process was compared. The first technique was used to analyze the composition of the natural and metal-loaded biomass, and additionally the composition of the solution before and after biosorption. The second technique was used to obtain a picture of the surface of natural and metal ion-loaded macroalgae, to map the elements on the cell wall of dry biomass, and to determine their concentration before and after biosorption. ICP-OES showed a better precision and lower detection limit than EDX, but SEM-EDX gave more information regarding the sample composition of Enteromorpha sp. Both techniques confirmed that biosorption is a surface phenomenon, in which alkali and alkaline earth metal ions were exchanged by metal ions from aqueous solution. Figure The advantages and disadvantages of ICP-OES and SEM-EDX techniques The Author(s), 2010 Biosorption nationallicence Microelement ions nationallicence Macroalga Enteromorpha sp nationallicence ICP-OES nationallicence SEM-EDX nationallicence Michalak Izabela Institute of Inorganic Technology and Mineral Fertilizers, Wrocław University of Technology, Smoluchowskiego 25, PL-50-372, Wrocław, Poland aut Chojnacka Katarzyna Institute of Inorganic Technology and Mineral Fertilizers, Wrocław University of Technology, Smoluchowskiego 25, PL-50-372, Wrocław, Poland aut Marycz Krzysztof Department of Hygiene and Ichtiology, Electron Microscope Laboratory, Environmental and Life Science University, Chełmońskiego 38c, PL-50-630, Wrocław, Poland aut Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 65-74 0026-3672 172:1-2<65 2011 172 604 https://doi.org/10.1007/s00604-010-0468-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0468-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michalak Izabela Institute of Inorganic Technology and Mineral Fertilizers, Wrocław University of Technology, Smoluchowskiego 25, PL-50-372, Wrocław, Poland aut NATIONALLICENCE 700 1- Chojnacka Katarzyna Institute of Inorganic Technology and Mineral Fertilizers, Wrocław University of Technology, Smoluchowskiego 25, PL-50-372, Wrocław, Poland aut NATIONALLICENCE 700 1- Marycz Krzysztof Department of Hygiene and Ichtiology, Electron Microscope Laboratory, Environmental and Life Science University, Chełmońskiego 38c, PL-50-630, Wrocław, Poland aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 65-74 0026-3672 172:1-2<65 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer