caa a22 4500 445879777 CHVBK 20180317145539.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00604-010-0479-x doi (NATIONALLICENCE)springer-10.1007/s00604-010-0479-x A third-generation hydrogen peroxide biosensor based on horseradish peroxidase cross-linked to multi-wall carbon nanotubes [Elektronische Daten] [Shuxia Xu, Xinfeng Zhang, Tao Wan, Chengxiao Zhang] A novel third generation biosensor for hydrogen peroxide (HP) was constructed by crosslinking horseradish peroxidase (HRP) onto an electrode modified with multi-wall carbon nanotubes (MWNTs). Glutaraldehyde and bovine serum albumin were used to cross-link HRP and the MWNTs. Scanning electron microscopy revealed that HRP was evenly distributed on the surface of the nanotubes. Factors influencing the performance of the biosensor were studied in detail. The biosensor exhibited a fast amperometric response (2.0s) to HP which is linear in the concentration range from 9.5mM to 0.95μM. The detection of limit is 0.4μM. The sensor exhibits good stability and durability (on testing 0.1mM solution of HP over a period of 10days), with a long-term relative standard deviation of <5%. Figure A third generation hydrogen peroxide biosensor was constructed by crosslinking horseradish peroxidase onto multi-wall carbon nanotubes modified electrode. The Multi-wall carbon nanotubes acted as "molecular wire” and the constructed biosensor exhibited a fast and strong amperometric response (2.0 s) to hydrogen peroxide Springer-Verlag, 2010 Horseradish peroxidase nationallicence Direct electron transfer nationallicence Multi-walled carbon nanotubes nationallicence Biosensor nationallicence Xu Shuxia College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 610059, Chengdu, China aut Zhang Xinfeng College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 610059, Chengdu, China aut Wan Tao College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 610059, Chengdu, China aut Zhang Chengxiao School of Chemical and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 199-205 0026-3672 172:1-2<199 2011 172 604 https://doi.org/10.1007/s00604-010-0479-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0479-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Shuxia College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 610059, Chengdu, China aut NATIONALLICENCE 700 1- Zhang Xinfeng College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 610059, Chengdu, China aut NATIONALLICENCE 700 1- Wan Tao College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 610059, Chengdu, China aut NATIONALLICENCE 700 1- Zhang Chengxiao School of Chemical and Materials Science, Shaanxi Normal University, 710062, Xi'an, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 199-205 0026-3672 172:1-2<199 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer