caa a22 4500 445879866 CHVBK 20180317145539.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00604-010-0476-0 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0476-0 Development of surface plasmon resonance immunosensor for the novel protein immunostimulating factor [Elektronische Daten] [Hongxia Chen, Jaebeom Lee, Wol-Soon Jo, Min-Ho Jeong, Kwangnak Koh] The immunostimulating factor (ISTF) plays an important role in immunopathologic changes associated with Actinobacillus actinomycetemcomitans where it is located in the outer cell membrane. We describe a novel method by which ISTF can be monitored using a protein chip system based on surface plasmon resonance (SPR). The affinity of ISTF to its monoclonal antibodies and other proteins was compared. In fabrication of an ISTF immunosensor, a calix[4]crown ether monolayer was anchored onto a gold surface for use as an artificial protein linker system, and then characterized by Fourier Transform infrared reflection absorption spectroscopy, atomic force microscopy, and cyclic voltammetry. Anti-ISTF was immobilized onto the monolayer, and the interaction between ISTF and its antibodies was investigated by SPR. The calix[4]crown ether was found to assemble as a monolayer on the gold surface. Orientation and accessibility of anti-ISTF were assessed by the selective binding of ISTF. Modification of the SPR chip with the calix[4]crown monolayer provides a reliable and simple experimental platform for investigation of isolated proteins under experimental conditions resembling those of their native environment. Figure Schematic diagram of sensor chip configuration Springer-Verlag, 2010 Immunostimulating factor (ISTF), Calixarene crown ether nationallicence Prolinker™ nationallicence Self-assembled monolayer (SAM) nationallicence Surface plasmon resonance (SPR) nationallicence Chen Hongxia School of Life Sciences, Shanghai University, 200444, Shanghai, People's Republic of China aut Lee Jaebeom College of Nanoscience and Nanotechnology, Pusan National University, 609-735, Pusan, South Korea aut Jo Wol-Soon College of Medicine, Dong-A University, 602-714, Pusan, South Korea aut Jeong Min-Ho College of Medicine, Dong-A University, 602-714, Pusan, South Korea aut Koh Kwangnak College of Nanoscience and Nanotechnology, Pusan National University, 609-735, Pusan, South Korea aut Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 171-176 0026-3672 172:1-2<171 2011 172 604 https://doi.org/10.1007/s00604-010-0476-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0476-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Hongxia School of Life Sciences, Shanghai University, 200444, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Lee Jaebeom College of Nanoscience and Nanotechnology, Pusan National University, 609-735, Pusan, South Korea aut NATIONALLICENCE 700 1- Jo Wol-Soon College of Medicine, Dong-A University, 602-714, Pusan, South Korea aut NATIONALLICENCE 700 1- Jeong Min-Ho College of Medicine, Dong-A University, 602-714, Pusan, South Korea aut NATIONALLICENCE 700 1- Koh Kwangnak College of Nanoscience and Nanotechnology, Pusan National University, 609-735, Pusan, South Korea aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 171-176 0026-3672 172:1-2<171 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer