caa a22 4500 445879920 CHVBK 20180317145539.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00604-010-0470-6 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0470-6 A hydrogen peroxide biosensor based on direct electron transfer from hemoglobin to an electrode modified with Nafion and activated nanocarbon [Elektronische Daten] [Jie Song, JiMing Xu, PuSu Zhao, LuDe Lu, JianChun Bao] A biosensor for hydrogen peroxide (HP) was developed by immobilizing hemoglobin on a glassy carbon electrode modified with activated carbon nanoparticles/Nafion. The characteristics of the sensor were studied by UV-vis spectroscopy and electrochemical methods. The immobilized Hb retained its native secondary structure, undergoes direct electron transfer (with a heterogeneous rate constant of 3.37 ± 0.5s−1), and displays excellent bioelectrocatalytic activity to the reduction of HP. Under the optimal conditions, its amperometric response varies linearly with the concentration of HP in the range from 0.9μM to 17μM. The detection limit is 0.4μM (at S/N = 3). Due to the commercial availability and low cost of activated carbon nanoparticles, it can be considered as a useful supporting material for construction of other third-generation biosensors. Figure A biosensor for hydrogen peroxide (HP) was developed by immobilizing hemoglobin on a glassy carbon electrode modified with activated carbon nanoparticles/Nafion. It can be considered as a useful supporting material for construction of other third-generation biosensors. Springer-Verlag, 2010 Hydrogen peroxide biosensor nationallicence AC/NF composite film nationallicence Hemoglobin nationallicence Direct electron transfer nationallicence Song Jie Materials Chemistry Laboratory, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu, People's Republic of China aut Xu JiMing Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, Huaiyin Normal University, 223300, Huaian, Jiangsu, People's Republic of China aut Zhao PuSu Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, Huaiyin Normal University, 223300, Huaian, Jiangsu, People's Republic of China aut Lu LuDe Materials Chemistry Laboratory, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu, People's Republic of China aut Bao JianChun Materials Chemistry Laboratory, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu, People's Republic of China aut Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 117-123 0026-3672 172:1-2<117 2011 172 604 https://doi.org/10.1007/s00604-010-0470-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0470-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Jie Materials Chemistry Laboratory, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Xu JiMing Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, Huaiyin Normal University, 223300, Huaian, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhao PuSu Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, Huaiyin Normal University, 223300, Huaian, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Lu LuDe Materials Chemistry Laboratory, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Bao JianChun Materials Chemistry Laboratory, Nanjing University of Science and Technology, 210094, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 172/1-2(2011-02-01), 117-123 0026-3672 172:1-2<117 2011 172 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer