caa a22 4500 445880031 CHVBK 20180317145539.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00604-011-0692-2 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0692-2 Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex [Elektronische Daten] [Zhanguang Chen, Sihua Qian, Guoliang Liu, Xi Chen, Junhui Chen] We have developed a resonance light scattering (RLS) quenching assay for the highly sensitive determination of doxorubicin (DOX) and daunorubicin (DAU). It is based on the reduction of the intensity of the shoulder of the RLS spectra at 443nm. The intensity of the RLS of the ethidium-DNA system decrease linearly on addition of trace quantities of DOX or DAU within the concentration range of 0.008 to 12.0μgmL−1 for DOX, and of 0.010 to 21.0μgmL−1 for DAU. The detection limits are 3.0 and 5.0ngmL−1, respectively. The assay was successfully applied to the determination of DAU in synthetic and serum samples. Compared to the reported methods for anthracyclines, this assay displays higher sensitivity, lower detection limits, and a wider linear range. Graphical abstract The addition of trace amount of drugs into the EB-DNA system can induce the decreased RLS intensity of EB-DNA system at the shoulder peak in BR buffer solution (pH 2.0). Besides, the decrement of RLS intensities was proportional to the concentration of drugs. Based on this phenomenon, a new RLS assay for the detection of anthracycline antibiotics was developed. Springer-Verlag, 2011 Resonance light scattering (RLS) nationallicence Shoulder peak nationallicence Doxorubicin (DOX) nationallicence Daunorubicin (DAU) nationallicence DNA nationallicence Chen Zhanguang Department of Chemistry, Shantou University, 515063, Shantou, China aut Qian Sihua Department of Chemistry, Shantou University, 515063, Shantou, China aut Liu Guoliang Department of Chemistry, Shantou University, 515063, Shantou, China aut Chen Xi Guangdong Pharmaceutical University, 510006, Guangzhou, China aut Chen Junhui Department of Radiology, Peking University Shenzhen Hospital, 518036, Shenzhen, China aut Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 217-223 0026-3672 175:3-4<217 2011 175 604 https://doi.org/10.1007/s00604-011-0692-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0692-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Zhanguang Department of Chemistry, Shantou University, 515063, Shantou, China aut NATIONALLICENCE 700 1- Qian Sihua Department of Chemistry, Shantou University, 515063, Shantou, China aut NATIONALLICENCE 700 1- Liu Guoliang Department of Chemistry, Shantou University, 515063, Shantou, China aut NATIONALLICENCE 700 1- Chen Xi Guangdong Pharmaceutical University, 510006, Guangzhou, China aut NATIONALLICENCE 700 1- Chen Junhui Department of Radiology, Peking University Shenzhen Hospital, 518036, Shenzhen, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 217-223 0026-3672 175:3-4<217 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer