caa a22 4500 445880074 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00604-011-0657-5 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0657-5 CdS quantum dots as a fluorescent sensing platform for nucleic acid detection [Elektronische Daten] [Wenbo Lu, Xiaoyun Qin, Yonglan Luo, Guohui Chang, Xuping Sun] We demonstrate that CdS quantum dots (QDs) can be applied to fluorescence-enhanced detection of nucleic acids in a two-step protocol. In step one, a fluorescently labeled single-stranded DNA probe is adsorbed on the QDs to quench its luminescence. In step two, the hybridization of the probe with its target ssDNA produces a double-stranded DNA which detaches from the QD. This, in turn, leads to the recovery of the fluorescence of the label. The lower detection limit of the assay is as low as 1nM. The scheme (that was applied to detect a target DNA related to the HIV) is simple and can differentiate between perfectly complementary targets and mismatches. Figure CdS quantum dots (CdSQDs) can serve as an effective sensing platform for fluorescence-enhanced DNA detection. This sensing system has a detection limit of 1nM and is capable of differentiating between complementary and mismatched sequences. Springer-Verlag, 2011 CdS quantum dots nationallicence DNA detection nationallicence Fluorescence nationallicence Lu Wenbo Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut Qin Xiaoyun Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut Luo Yonglan Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut Chang Guohui Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut Sun Xuping Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 355-359 0026-3672 175:3-4<355 2011 175 604 https://doi.org/10.1007/s00604-011-0657-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0657-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Wenbo Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut NATIONALLICENCE 700 1- Qin Xiaoyun Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut NATIONALLICENCE 700 1- Luo Yonglan Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut NATIONALLICENCE 700 1- Chang Guohui Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut NATIONALLICENCE 700 1- Sun Xuping Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, 637002, Nanchong, Sichuan, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 355-359 0026-3672 175:3-4<355 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer