caa a22 4500 445880090 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00604-011-0682-4 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0682-4 A comparison of horseradish peroxidase, gold nanoparticles and qantum dots as labels in non-instrumental gel-based immunoassay [Elektronische Daten] [Natalia Beloglazova, Irina Goryacheva, Reinhard Niessner, Dietmar Knopp] We have developed a column gel-based immunoassays for the model analyte benzo[a]pyrene (B[a]P), and have evaluated three different kinds of labels, i.e., horseradish peroxidase, (HRP), colloidal gold (AuNPs), and quantum dots (QDs) with respect to rapid visual on-site testing. In case of HRP, a derivative of B[a]P-BA (benzo[a]pyrenebutyric acid) was directly coupled to HRP. In case of QDs and AuNPs, protein conjugates of B[a]P-BA were synthesized and used for surface functionalization. With the latter, previous coverage of the gold surface with 11-mercaptoundecanoic acid turned out to be advantageous in terms of antibody recognition and of preventing nonspecific binding to the gel layer. The assays based on the use of particle labels requires 4 consecutive working steps only, while those based on HRP require 5 additions of reagent. The lower limit of detection for B[a]P is 5ngL−1 in case of using HPR or QDs as a label, but 25ngL−1 when using AuNPs. We believe that the use of QDs constitutes the most promising label for qualitative and semi-quantitative gel-based immunoassays. Figure We have developed column gel-based immunoassays for the model analyte benzo[a]pyrene (B[a]P) and have evaluated three different kinds of labels, i.e., horseradish peroxidase, colloidal gold and quantum dots with respect to rapid visual on-site testing. Springer-Verlag, 2011 Rapid test nationallicence Immunoassay nationallicence Enzyme nationallicence Gold nanoparticles nationallicence Quantum dots nationallicence benzo[a]pyrene nationallicence Beloglazova Natalia Department of General Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia aut Goryacheva Irina Department of General Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia aut Niessner Reinhard Chair for Analytical Chemistry, Institute of Hydrochemistry and Chemical Balneology, Technical University München, Marchioninistrasse 17, 81377, Munich, Germany aut Knopp Dietmar Chair for Analytical Chemistry, Institute of Hydrochemistry and Chemical Balneology, Technical University München, Marchioninistrasse 17, 81377, Munich, Germany aut Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 361-367 0026-3672 175:3-4<361 2011 175 604 https://doi.org/10.1007/s00604-011-0682-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0682-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beloglazova Natalia Department of General Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia aut NATIONALLICENCE 700 1- Goryacheva Irina Department of General Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia aut NATIONALLICENCE 700 1- Niessner Reinhard Chair for Analytical Chemistry, Institute of Hydrochemistry and Chemical Balneology, Technical University München, Marchioninistrasse 17, 81377, Munich, Germany aut NATIONALLICENCE 700 1- Knopp Dietmar Chair for Analytical Chemistry, Institute of Hydrochemistry and Chemical Balneology, Technical University München, Marchioninistrasse 17, 81377, Munich, Germany aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 361-367 0026-3672 175:3-4<361 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer