caa a22 4500 445880104 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00604-011-0669-1 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0669-1 Electrochemiluminescent detection of the hybridization of oligonucleotides using an electrode modified with nanocomposite of carbon nanotubes and gold nanoparticles [Elektronische Daten] [Haihong Chu, Jilin Yan, Yifeng Tu] We report on an electrochemiluminescent (ECL) sensing technique for the detection of the hybridization between oligonucleotides. A glassy carbon electrode was first functionalized with a composite prepared from gold nanoparticles and carbon nanotubes, and a sensor was then constructed by immobilizing the probing oligonucleotide. The ECL of luminol acts as the sensing signal. It is quenched, to a different degree, by the hybridized double strands of the oligonucleotide depending on the match status. The slope of the ECL response as a function of the status of hybridization drops with increasingly matched hybridization. The response is attributed to the interaction between luminol and the strands of oligomers, and also related to the reduction of reactive oxygen species. Figure An electrochemiluminescent sensing technique is developed by immobilization of probing oligonucleotide on a previously functionalized electrode with composite of carbon nanotubes and gold nano-particles. The quenching efficiency for ECL of luminol upon the hybridized status of target oligonucleotide with probing one had been detected where the mismatch ratio was distinguishable. ECL response for luminol on (a) CNTs/AuNP/GCE, (b) probe-strand/CNTs /AuNP/GCE, (c) 1-3 hybridized strands/CNTs/AuNP/GCE and (d) 1-2 hybridized strands/CNTs/AuNP/GCE. Springer-Verlag, 2011 Oligonucleotide hybridization nationallicence Electrochemiluminescent sensing nationallicence Luminol nationallicence Chemically modified electrode nationallicence Nano-composite nationallicence Chu Haihong Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, Suzhou Industrial Park, 215123, Suzhou, People's Republic of China aut Yan Jilin Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, Suzhou Industrial Park, 215123, Suzhou, People's Republic of China aut Tu Yifeng Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, Suzhou Industrial Park, 215123, Suzhou, People's Republic of China aut Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 209-216 0026-3672 175:3-4<209 2011 175 604 https://doi.org/10.1007/s00604-011-0669-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0669-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chu Haihong Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, Suzhou Industrial Park, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yan Jilin Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, Suzhou Industrial Park, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tu Yifeng Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, Suzhou Industrial Park, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/3-4(2011-12-01), 209-216 0026-3672 175:3-4<209 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer