caa a22 4500 445880252 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0654-8 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0654-8 Rapid detection of Staphylococcus aureus via a sensitive DNA hybridization assay based on a long-lifetime luminescent europium marker [Elektronische Daten] [Min Ruan, Cheng-Gang Niu, Guang-Ming Zeng, Pin-Zhu Qin, Xiao-Yu Wang, Da-Wei Huang, Jing Huang, Chang-Zheng Fan] A two-probe tandem nucleic acid hybridization assay for detection of Staphylococcus aureus is presented. It is based on a europium(III) complex as a marker that has a long fluorescence lifetime, high quantum yield and can be easily conjugated to an oligonucleotide signaling probe. The amino-modified capture probe was associated with the signaling probe to form a two-probe tandem DNA pattern that is complementary to the target DNA. The method was optimized in terms of hybridization temperature, hybridization time and washing time. This resulted in good specificity and sensitivity when detecting such bacteria in food samples. Figure A europium complex as a long fluorescent lifetime marker was conjugated to an oligonucleotide. The amino-modified capture probe was associated with the signaling probe to form a two-probe tandem DNA pattern that is complementary to the target DNA. The results are shown that this method has good specificity and sensitivity. Springer-Verlag, 2011 Staphylococcus aureus nationallicence Capture probe nationallicence Signaling probe nationallicence Long lifetime biomarker nationallicence DNA hybridization nationallicence Pathogenic microorganisms monitoring nationallicence Ruan Min College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Niu Cheng-Gang College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Zeng Guang-Ming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Qin Pin-Zhu College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Wang Xiao-Yu College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Huang Da-Wei College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Huang Jing College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Fan Chang-Zheng College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 105-112 0026-3672 175:1-2<105 2011 175 604 https://doi.org/10.1007/s00604-011-0654-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0654-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruan Min College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Niu Cheng-Gang College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Zeng Guang-Ming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Qin Pin-Zhu College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Wang Xiao-Yu College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Huang Da-Wei College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Huang Jing College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Fan Chang-Zheng College of Environmental Science and Engineering, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 105-112 0026-3672 175:1-2<105 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer