caa a22 4500 445880279 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0637-9 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0637-9 Dendrimer-based biosensor for chemiluminescent detection of DNA hybridization [Elektronische Daten] [Pei Liu, Xu Hun, Hongqing Qing] We report on a highly sensitive chemiluminescent (CL) biosensor for the sequenc-specific detection of DNA using a novel bio barcode DNA probe modified with gold nanoparticles that were covered with a dendrimer. The modified probe is composed of gold nanoparticles, a dendrimer, the CL reagent, and the DNA. The capture probe DNA was immobilized on magnetic beads covered with gold. It first hybridizes with the target DNA and then with one terminal end of the signal DNA on the barcoded DNA probe. CL was generated by adding H2O2 and Co(II) ions as the catalyst. The immobilization of dendrimer onto the gold nanoparticles can significantly enhance sensitivity and gives a detection limit of 6fmol L-1 of target DNA. Graphical Abstract A sensitive chemiluminescent biosensor for the sequenc-specific detection of DNA using a novel bio barcode DNA probe modified with gold nanoparticle that were covered with a dendrimer was reported. The immobilization of dendrimer onto the gold nanoparticles enhances sensitivity and gives a detection limit of 6fM of target DNA. Springer-Verlag, 2011 Dendrimer nationallicence DNA biosensor nationallicence Chemiluminescent nationallicence Gold nanoparticle nationallicence Liu Pei School of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, 710021, Xi an, People's Republic of China aut Hun Xu Shandong Provincial Key Laboratory Of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 266042, Qingdao, People's Republic of China aut Qing Hongqing Shandong Provincial Key Laboratory Of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 266042, Qingdao, People's Republic of China aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 201-207 0026-3672 175:1-2<201 2011 175 604 https://doi.org/10.1007/s00604-011-0637-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0637-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Pei School of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, 710021, Xi an, People's Republic of China aut NATIONALLICENCE 700 1- Hun Xu Shandong Provincial Key Laboratory Of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 266042, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Qing Hongqing Shandong Provincial Key Laboratory Of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 266042, Qingdao, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 201-207 0026-3672 175:1-2<201 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer