caa a22 4500 445880309 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0638-8 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0638-8 Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials [Elektronische Daten] [Ying Wang, Kun Qian, Kai Guo, Jilie Kong, Jean-Louis Marty, Chengzhong Yu, Baohong Liu] An amperometric biosensor for hydrogen peroxide (H2O2) has been constructed by immobilizing cytochrome c on an indium/tin oxide (ITO) electrode modified with a macroporous material. Cyclic voltammetry showed that the direct and quasi-reversible electron transfer of cytochrome c proceeds without the need for an electron mediator. A surface-controlled electron transfer process can be observed with an apparent heterogeneous electron-transfer rate constant (ks) of 29.2s−1. The biosensor displays excellent electrocatalytic responses to the reduction of H2O2 to give amperometric responses that increase steadily with the concentration of H2O2 in the range from 5μM to 2mM. The detection limit is 0.61μM at pH7.4. The apparent Michaelis-Menten constant (Km) of the biosensor is 1.06mM. This investigation not only provided a method for the direct electron transfer of cytochrome c on macroporous materials, but also established a feasible approach for durable and reliable detection of H2O2. Figure Biosensor for hydrogen peroxide was developed by immobilizing cytochrome c in the macroporous ordered silica foam (MOSF) through the electrostatic interaction. The achievement of the direct electron transfer between cytochrome c and electrode surface indicated that the MOSF modified electrode displayed good affinity and biocompatibility for cytochrome c. Springer-Verlag, 2011 Macroporous material nationallicence Cytochrome nationallicence Hydrogen peroxide nationallicence Direct electron transfer nationallicence Biosensor nationallicence Wang Ying Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut Qian Kun ARC Centre of Excellence for Functional Nanomaterials and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, Brisbane, QLD, Australia aut Guo Kai Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut Kong Jilie Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut Marty Jean-Louis IMAGES EA 4218 Centre de Phytopharmacie, Université de Perpignan, 52 Avenue Paul Alduy, F-66860, Perpignan Cedex, France aut Yu Chengzhong ARC Centre of Excellence for Functional Nanomaterials and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, Brisbane, QLD, Australia aut Liu Baohong Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 87-95 0026-3672 175:1-2<87 2011 175 604 https://doi.org/10.1007/s00604-011-0638-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0638-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Ying Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Qian Kun ARC Centre of Excellence for Functional Nanomaterials and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Guo Kai Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Kong Jilie Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Marty Jean-Louis IMAGES EA 4218 Centre de Phytopharmacie, Université de Perpignan, 52 Avenue Paul Alduy, F-66860, Perpignan Cedex, France aut NATIONALLICENCE 700 1- Yu Chengzhong ARC Centre of Excellence for Functional Nanomaterials and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Liu Baohong Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 87-95 0026-3672 175:1-2<87 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer