caa a22 4500 445880325 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0668-2 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0668-2 Determination of some trace elements by flame atomic absorption spectrometry after preconcentration and separation by Escherichia coli immobilized on multiwalled carbon nanotubes [Elektronische Daten] [Nihat Aydemir, Nilgun Tokman, Alper Akarsubasi, Asli Baysal, Süleyman Akman] We have immobilized living and non-living Escherichia coli (E. coli) bacteria on multiwalled carbon nanotubes (MWCNT) and used such materials as a biosorbent for the separation and preconcentration of copper, cobalt, cadmium and nickel prior to their determination by flame atomic absorption spectrometry (FAAS). E. coli bacteria cells were mixed with MWCNTs in a 1:1 ratio, dried and placed at the tip of a 50-mL syringe. The ions were retained on the sorbent and then eluted by drawing and ejecting back the sample (or standard solution) and an eluent, respectively. The effects of various experimental parameters on the sorption and elution were investigated. The analytes were quantitatively retained (at pH values of 7) and eluted (with 0.5M nitric acid) with high precision, the RSD being <5%. The performances of the new sorbents were compared using certified reference materials. The sorbent modified with living E. coli has a higher adsorption capacity and displays somewhat better recoveries compared to sorbent based on non-living E. coli. Both sorbents were successfully used for the separation and preconcentration of copper, cobalt, cadmium and nickel prior to their determination by flame atomic absorption spectrometry. Figure SEM photograph of (a) living E. coli immobilized on MWCNT and (b) non-living E. coli immobilized on MWCNT Springer-Verlag, 2011 Preconcentration nationallicence Separation nationallicence Flame atomic absorption spectrometry nationallicence Escherichia coli nationallicence Biosorbtion nationallicence Multiwalled carbon nanotubes nationallicence Aydemir Nihat Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut Tokman Nilgun Scientific and Technical Research Council of Turkey (TUBITAK), National Metrology Institute, PK 54 41470, Gebze, Kocaeli, Turkey aut Akarsubasi Alper Faculty of Science & Letters, Department of Molecular Biology and Genetics, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut Baysal Asli Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut Akman Süleyman Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 185-191 0026-3672 175:1-2<185 2011 175 604 https://doi.org/10.1007/s00604-011-0668-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0668-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aydemir Nihat Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut NATIONALLICENCE 700 1- Tokman Nilgun Scientific and Technical Research Council of Turkey (TUBITAK), National Metrology Institute, PK 54 41470, Gebze, Kocaeli, Turkey aut NATIONALLICENCE 700 1- Akarsubasi Alper Faculty of Science & Letters, Department of Molecular Biology and Genetics, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut NATIONALLICENCE 700 1- Baysal Asli Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut NATIONALLICENCE 700 1- Akman Süleyman Faculty of Science & Letters, Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 185-191 0026-3672 175:1-2<185 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer