caa a22 4500 445880341 CHVBK 20180317145540.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0665-5 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0665-5 A glassy carbon electrode modified with graphene and tyrosinase immobilized on platinum nanoparticles for sensing organophosphorus pesticides [Elektronische Daten] [Tao Liu, Minrong Xu, Huanshun Yin, Shiyun Ai, Xiangjin Qu, Shanshan Zong] An amperometric biosensor is described for the detection of organophosphorus pesticides. It is based on the enzyme tyrosinase immobilized on platinum nanoparticles and the use of a glassy carbon electrode modified with graphene. Tyrosinase was immobilized on the electrode surface via electrostatic interaction between a monolayer of cysteamine and the enzyme. In the presence of catechol as a substrate, the pesticides chlorpyrifos, profenofos and malathion can be determined as a result of their inhibition of the enzyme which catalyzes the oxidation of catechol to o-quinone. Platinum nanoparticles and graphene effectively enhance the efficiency of the electrochemical reduction of o-quinone, thus improving sensitivity. Under optimum experimental conditions, the inhibition effect of the pesticides investigated is proportional to their concentrations in the lower ppb-range. The detection limits are 0.2, 0.8 and 3ppb for chlorpyrifos, profenofos and malathion, respectively. The biosensor displays good repeatability and acceptable stability. Figure A tyrosinase-based biosensor was developed for determining organophosphorus pesticides. The biosensor owned high sensitivity by combining platinum nanoparticles and graphene, and the immobilized tyrosinase had a great affinity to catechol. Low detection limits and reasonable liner ranges were obtained. The biosensor also displayed good repeatability and acceptable stability. Springer-Verlag, 2011 Amperometric biosensor nationallicence Tyrosinase nationallicence Platinum nanoparticles nationallicence Graphene nationallicence Organophosphorus pesticides nationallicence Liu Tao College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut Xu Minrong College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut Yin Huanshun College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut Ai Shiyun College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut Qu Xiangjin College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut Zong Shanshan College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 129-135 0026-3672 175:1-2<129 2011 175 604 https://doi.org/10.1007/s00604-011-0665-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0665-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Tao College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut NATIONALLICENCE 700 1- Xu Minrong College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut NATIONALLICENCE 700 1- Yin Huanshun College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut NATIONALLICENCE 700 1- Ai Shiyun College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut NATIONALLICENCE 700 1- Qu Xiangjin College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut NATIONALLICENCE 700 1- Zong Shanshan College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 129-135 0026-3672 175:1-2<129 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer