caa a22 4500 445880384 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0645-9 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0645-9 Visualization of red-ox proteins on the gold surface using enzymatic polypyrrole formation [Elektronische Daten] [Almira Ramanaviciene, Asta Kausaite-Minkstimiene, Yasemin Oztekin, Geta Carac, Jaroslav Voronovic, Natalija German, Arunas Ramanavicius] We describe a new method for the visualization of the activity of red-ox proteins on a gold interface. Glucose oxidase was selected as a model system. Surfaces were modified by adhesion of glucose oxidase on (a) electrochemically cleaned gold; (b) gold films modified with gold nanoparticles, (c) a gold surface modified with self-assembled monolayer, and (d) covalent immobilization of protein on the gold surface modified with a self-assembled monolayer. The simple optical method for the visualization of enzyme on the surfaces is based on the enzymatic formation of polypyrrole. The activity of the enzyme was quantified via enzymatic formation of polypyrrole, which was detected and investigated by quartz microbalance and amperometric techniques. The experimental data suggest that the enzymatic formation of the polymer may serve as a method to indicate the adhesion of active redox enzyme on such surfaces. Figure An optical method for the evaluation of activity and distribution of glucose oxidase on the different surfaces was described. The enzymatic synthesis of polypyrrole (black colour) was successfully applied for the visualization of active enzyme on the surfaces. Springer-Verlag, 2011 Glucose oxidase nationallicence Polypyrrole nationallicence Enzymatic synthesis nationallicence Self-assembled monolayer nationallicence Gold surface nationallicence Ramanaviciene Almira Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Kausaite-Minkstimiene Asta Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Oztekin Yasemin Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Carac Geta Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Voronovic Jaroslav Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut German Natalija Division of Immunotechnology, State Research Institute Center for Innovative Medicine, Zygimantu 9, 01102, Vilnius, Lithuania aut Ramanavicius Arunas Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 79-86 0026-3672 175:1-2<79 2011 175 604 https://doi.org/10.1007/s00604-011-0645-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0645-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ramanaviciene Almira Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Kausaite-Minkstimiene Asta Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Oztekin Yasemin Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Carac Geta Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Voronovic Jaroslav Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- German Natalija Division of Immunotechnology, State Research Institute Center for Innovative Medicine, Zygimantu 9, 01102, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Ramanavicius Arunas Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 79-86 0026-3672 175:1-2<79 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer