caa a22 4500 445880406 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00604-011-0641-0 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0641-0 A biopolymer-based carbon nanotube interface integrated with a redox shuttle and a D-sorbitol dehydrogenase for robust monitoring of D-sorbitol [Elektronische Daten] [Jana Šefčovičová, Jaroslav Filip, Peter Tomčík, Peter Gemeiner, Marek Bučko, Peter Magdolen, Jan Tkac] We describe the preparation and characterization of a glassy carbon electrode modified with a bionanocomposite consisting of a hyaluronic acid, dispersed carbon nanotubes, and electrostatically bound toluidine blue. The electrode was used to detect NADH in the batch and flow-injection mode of operation. The electrode was further modified by immobilizing sorbitol dehydrogenase to result in biosensor for D-sorbitol that displays good operational stability, a sensitivity of 10.6μAmM−1cm−2, a response time of 16s, and detection limit in the low micromolar range. The biosensor was successfully applied to off-line monitoring of D-sorbitol during its bioconversion into L-sorbose (a precursor in the synthesis of vitamin C) by Gluconobacter oxydans. The sample assay precision is 2.5% (an average RSD) and the throughput is 65h−1 if operated in the flow-injection mode. The validation of this biosensor against a reference HPLC method resulted in a slope of correlation of 1.021 ± 0.001 (R 2 = 0.99997). Figure Immobilisation of D-sorbitol dehydrogenase between two biopolymers on carbon nanotube layer provides stable and robust D-sorbitol biosensing with a mediator being electrostatically bound within the matrix. The biosensor was succesfully applied in analysis of fermentation samples with througput of assays of 65h−1 in flow system. Springer-Verlag, 2011 Biosensor nationallicence Carbon nanotubes nationallicence Hyaluronic acid nationallicence NADH sensor nationallicence D-sorbitol dehydrogenase nationallicence Šefčovičová Jana Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut Filip Jaroslav Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut Tomčík Peter Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-812 37, Bratislava, Slovakia aut Gemeiner Peter Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut Bučko Marek Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut Magdolen Peter Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut Tkac Jan Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 21-30 0026-3672 175:1-2<21 2011 175 604 https://doi.org/10.1007/s00604-011-0641-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0641-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Šefčovičová Jana Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Filip Jaroslav Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Tomčík Peter Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-812 37, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Gemeiner Peter Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Bučko Marek Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Magdolen Peter Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Tkac Jan Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 175/1-2(2011-10-01), 21-30 0026-3672 175:1-2<21 2011 175 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer