caa a22 4500 445880422 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0584-5 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0584-5 Simultaneous detection of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes using oscillatory-flow multiplex PCR [Elektronische Daten] [Haiying Wang, Chunsun Zhang, Da Xing] An oscillatory-flow multiplex PCR method in a capillary microfluidic channel has been developed for the simultaneous determination of pre-purified DNA of multiple foodborne bacterial pathogens. The PCR solution passes three temperature zones in an oscillatory manner. The thermal stability and sample evaporation of the microfluidic device were investigated. Under controlled conditions, a highly efficient multiplex PCR was accomplished as demonstrated for the simultaneous amplifications of 278bp, 168bp, and 106bp DNA fragments within 35min after 35 cycles for simultaneous detection of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes. This is much shorter than that of a conventional PCR machine. The detection limits of bacterial genome DNA for the three species are about 399, 314, and 626 copies per μL, respectively. This is comparable to those obtained with the conventional multiplex PCR. Consequently, the oscillatory-flow multiplex PCR technology holds good potential for rapid amplification and detection of nucleic acids of microbial foodborne pathogens. Figure By using an oscillatory-flow multiplex PCR technology, the simultaneous amplifications of 278bp, 168bp, and 106bp DNA fragments can be achieved in 35min for detection of S. enterica, E. coli O157:H7, and L. monocytogenes. This is much shorter than that of a conventional multiplex PCR. Springer-Verlag, 2011 Microfluidics nationallicence Oscillatory-flow nationallicence Multiplex polymerase chain reaction nationallicence Foodborne bacterial pathogens nationallicence Wang Haiying MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou, China aut Zhang Chunsun MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou, China aut Xing Da MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou, China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 503-512 0026-3672 173:3-4<503 2011 173 604 https://doi.org/10.1007/s00604-011-0584-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0584-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Haiying MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Chunsun MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Xing Da MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 503-512 0026-3672 173:3-4<503 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer