caa a22 4500 445880430 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0573-8 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0573-8 Polyvinyl butyral nanobeads: preparation, characterization, biocompatibility and cancer cell uptake [Elektronische Daten] [Damir Posavec, André Dorsch, Udo Bogner, Günther Bernhardt, Stefan Nagl] We describe the synthesis of spherical poly(vinyl butyral) (PVB) nanobeads by controlled precipitation via addition of non-solvent. Effects of various reaction parameters on nanoparticle size were investigated by dynamic light scattering and electron microscopy. The ability to incorporate dopant molecules was studied using a fluorescent perylene derivative as a model additive, and the dye-doped nanoparticles were investigated by confocal microscopy. In an optimized experimental protocol, PVB nanoparticles were obtained that were efficiently taken up by human cancer cells devoid of coating. The novel nanospheres are economic, easy to prepare and capable of incorporating additives. Lacking cytotoxicity in vitro, PVB nanobeads are attractive with respect to various potential applications such as optical imaging and particle tracking, diagnostics, and drug delivery. Figure The synthesis and characterization of polyvinyl butyral nanoparticles is described. The beads were doped with a dye and used for intracellular fluorescence imaging. The nanospheres are efficiently taken up without coating and do not display in vitro cytotoxicity on human cancer cells. They are therefore attractive for various applications. Springer-Verlag, 2011 Polyvinyl butyral nationallicence Nanoparticles nationallicence Fluorescent microbeads nationallicence Live cell imaging nationallicence Cytotoxicity nationallicence Posavec Damir Institut für Physikalische Chemie, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut Dorsch André Institut für Analytische Chemie, Universität Leipzig, Linnéstr. 3, 04103, Leipzig, Germany aut Bogner Udo Institut für Physikalische Chemie, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut Bernhardt Günther Institut für Pharmazeutische/Medizinische Chemie II, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut Nagl Stefan Institut für Analytische Chemie, Universität Leipzig, Linnéstr. 3, 04103, Leipzig, Germany aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 391-399 0026-3672 173:3-4<391 2011 173 604 https://doi.org/10.1007/s00604-011-0573-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0573-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Posavec Damir Institut für Physikalische Chemie, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Dorsch André Institut für Analytische Chemie, Universität Leipzig, Linnéstr. 3, 04103, Leipzig, Germany aut NATIONALLICENCE 700 1- Bogner Udo Institut für Physikalische Chemie, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Bernhardt Günther Institut für Pharmazeutische/Medizinische Chemie II, Universität Regensburg, Universitätsstr. 31, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Nagl Stefan Institut für Analytische Chemie, Universität Leipzig, Linnéstr. 3, 04103, Leipzig, Germany aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 391-399 0026-3672 173:3-4<391 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer