caa a22 4500 445880457 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0544-0 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0544-0 Simple construction of an enzymatic glucose biosensor based on a nanocomposite film prepared in one step from iron oxide, gold nanoparticles, and chitosan [Elektronische Daten] [Jingjing Li, Ruo Yuan, Yaqin Chai] The one-step synthesis is reported of a nanofilm composed of iron oxide and gold nanoparticles in a chitosan matrix that can act as a novel matrix for the immobilization of glucose oxidase (GOx) to fabricate a glucose biosensor. The use for the composite film strongly increased the effective electrode surface for loading of GOx. The size and shape of the iron oxide nanoparticles were examined by transmission electron micrograph. Direct electron transfer and electrocatalysis by GOx was investigated via cyclic voltammetry and chronoamperometry. Under optimized conditions, the biosensor has a response time of 6s and a linear response in the range between 3μM and 0.57mM of glucose, with a detection limit of 1.2μM at a signal-to-noise ratio of 3. This novel and disposable mediatorless glucose biosensor may form the basis for a future mass-produced glucose biosensor. Figure In this paper, based on the direct electrochemistry of redox enzyme, we try to integrate the excellent properties of iron oxide-gold nanoparticle-chitosan composite film with the advantages of one-step electrodeposition to fabricate a sensitive and stable glucose biosensor. Springer-Verlag, 2011 Electrochemical biosensor nationallicence Direct electron transfer nationallicence Iron oxide-gold nanoparticles-chitosan composite film nationallicence Glucose oxidase nationallicence Glucose biosensor nationallicence Li Jingjing Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, People's Republic of China aut Yuan Ruo Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, People's Republic of China aut Chai Yaqin Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, People's Republic of China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 369-374 0026-3672 173:3-4<369 2011 173 604 https://doi.org/10.1007/s00604-011-0544-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0544-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jingjing Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Ruo Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Chai Yaqin Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 369-374 0026-3672 173:3-4<369 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer