caa a22 4500 445880465 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0558-7 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0558-7 Sensitive DNA-hybridization biosensors based on gold nanoparticles for testing DNA damage by Cd(II) ions [Elektronische Daten] [Qianru Zhang, Panpan Dai, Zhousheng Yang] A DNA biosensor was constructed by immobilizing a 20-mer oligonucleotide probe and hybridizing it with its complementary oligomer on the surface of a glassy carbon electrode modified with gold nanoparticles. The properties of the biosensor and its capability of recognizing its complementary sequence were studied by electrochemical impedance spectroscopy. The oxidative stress caused by cadmium ions can be monitored by differential pulse voltammetry using the cobalt(III)tris(1,10-phenanthroline) complex and methylene blue as electrochemical indicators. The biosensor is capable of indicating damage caused by Cd(II) ions in pH 6.0 solution. The results showed that the biosensor can be used for rapid screening for DNA damage. Figure DPV of DNA biosensors before (a, c) and after hybridization (b, d) at 1.0 ×10-7 mol·L-1target DNA concentration, (a) probe DNA/Au/GCE and (b) dsDNA/Au/GCE (c) probe DNA/GCE, (d) dsDNA/GCE Springer-Verlag, 2011 DNA-hybridization biosensor nationallicence DNA damage nationallicence Cd(II) ion nationallicence Co(phen)33+ nationallicence Methylene blue nationallicence Zhang Qianru Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, People's Republic of China aut Dai Panpan Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, People's Republic of China aut Yang Zhousheng Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, People's Republic of China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 347-352 0026-3672 173:3-4<347 2011 173 604 https://doi.org/10.1007/s00604-011-0558-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0558-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Qianru Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, People's Republic of China aut NATIONALLICENCE 700 1- Dai Panpan Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Zhousheng Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, 241000, Wuhu, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 347-352 0026-3672 173:3-4<347 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer