caa a22 4500 44588052X CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0559-6 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0559-6 Direct electrochemistry of hemoglobin immobilized on CdS:Mn nanoparticles [Elektronische Daten] [Zhong-Qin Pan, Hong Fan, Chuan-Guo Shi, Ning Bao, Chun-Mei Yu, Hai-Ying Gu] Hemoglobin (Hb) was immobilized on CdS:Mn nanoparticles (NPs) capped with citrate to obtain an amperometric biosensor for determination of hydrogen peroxide (H2O2). The NPs were prepared from aqueous solution via a precipitation method. The UV-vis spectra of the film with Hb on the NPs and of free Hb solutions suggested that Hb retained its bioactivity and native conformation in the film. In buffer of pH7.0, the sensor film exhibited a pair of quasi-reversible redox peaks originating from the Hb-heme Fe(III)/Fe(II) redox couple. The formal potential of Hb varied linearly with the increase of pH from 3.0 to 9.0 with a slope of −46mV·pH−1, this indicating that one proton participates in the redox reaction. The electron transfer rate constant (ks) is 2.2 s−1, suggesting that the NPs provide a benign microenvironment for Hb to undergo direct electron transfer. Despite the absence of an electron mediator, the biosensor displays an analytical performance which is better than any others described in previous reports. Hence, the NPs may provide a simple and alternative approach towards electrochemical biosensors. Figure The CdS:Mn NPs capped with citrate may provide a benign microenvironment for Hb to undergo direct electron transfer so that the electrochemical detection could be improved. Springer-Verlag, 2011 Hemoglobin nationallicence CdS:Mn nanoparticles nationallicence Biosensor nationallicence Hydrogen peroxide nationallicence Direct electron transfer nationallicence Pan Zhong-Qin Institute of Nautical Medicine, Nantong University, 226001, Nantong, People's Republic of China aut Fan Hong Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut Shi Chuan-Guo Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut Bao Ning Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut Yu Chun-Mei Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut Gu Hai-Ying Institute of Nautical Medicine, Nantong University, 226001, Nantong, People's Republic of China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 277-283 0026-3672 173:3-4<277 2011 173 604 https://doi.org/10.1007/s00604-011-0559-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0559-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pan Zhong-Qin Institute of Nautical Medicine, Nantong University, 226001, Nantong, People's Republic of China aut NATIONALLICENCE 700 1- Fan Hong Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut NATIONALLICENCE 700 1- Shi Chuan-Guo Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut NATIONALLICENCE 700 1- Bao Ning Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut NATIONALLICENCE 700 1- Yu Chun-Mei Institute of Analytical Chemistry for Life Science, Nantong University, 226019, Nantong, People's Republic of China aut NATIONALLICENCE 700 1- Gu Hai-Ying Institute of Nautical Medicine, Nantong University, 226001, Nantong, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 277-283 0026-3672 173:3-4<277 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer