caa a22 4500 445880562 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0557-8 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0557-8 Label-free immunosensing of microcystin-LR using a gold electrode modified with gold nanoparticles [Elektronische Daten] [Ping Tong, Shurong Tang, Yu He, Yanhong Shao, Lan Zhang, Guonan Chen] The hepatotoxic microcystins, especially microcystin-LR (MC-LR), are causing serious problems to public health and fisheries. We describe here a label-free amperometric immunosensor for rapid determination of MC-LR in water sample. The sensor was prepared by immobilizing antibody on a gold electrode coated with L-cysteine-modified gold nanoparticles. The stepwise self-assembly of the immunosensor was monitored and characterized by means of electrochemical impedance spectroscopy and differential pulse voltammetry. A 0.60mmol L−1 solution of hydroquinone was used as the electron mediator. The immunosensor was incubated with MC-LR at 25°C for 20min, upon which the differential pulse voltammetric current changed linearly over the concentration range from 0.05 to 15.00μg L−1, with a detection limit of 20ng L−1. The developed biosensor was used to determine MC-LR in spiked crude algae samples. The recovery was in the range from 95.6 to 105%. This method is simple, economical and efficient, this making it potentially suitable for field analysis of MC-LR in crude algae and water samples. Figure The present investigation combines SAM monolayer with gold nanoparticles monolayer to prepare a stable film to immobilize the antibody, and takes hydroquinone as electron mediator, establishes a miniature, economic, compatible and label-free amperometric immunosensor for the quick detection of MC-LR. Springer-Verlag, 2011 Amperometric immunosensor nationallicence Label-free nationallicence Microcystin nationallicence Gold nanoparticle nationallicence Tong Ping Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut Tang Shurong College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut He Yu College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut Shao Yanhong College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut Zhang Lan Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut Chen Guonan College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 299-305 0026-3672 173:3-4<299 2011 173 604 https://doi.org/10.1007/s00604-011-0557-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0557-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tong Ping Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- Tang Shurong College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- He Yu College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- Shao Yanhong College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- Zhang Lan Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- Chen Guonan College of Chemistry and Chemical Engineering, Fuzhou University, 523 Industrial Road, 350002, Fuzhou, Fujian, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 299-305 0026-3672 173:3-4<299 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer