caa a22 4500 445880570 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0572-9 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0572-9 Multilayer structured immunosensor based on a glassy carbon electrode modified with multi-wall carbon nanotubes, polythionine, and gold nanoparticles [Elektronische Daten] [Qin Mi, Zheng Wang, Chun Chai, Juan Zhang, Bo Zhao, Chang Chen] An immunosensor for determination of salbutamol was developed. It based on glass carbon electrode (GCE) modified with a conductive multilayer film comprised of multi-wall carbon nanotubes, polythionine and gold nanoparticles. Salbutamol antibody was immobilized on the surface of the modified GCE which then was blocked with bovine serum albumin (BSA). The stepwise self-assembly process of the immunosensor was studied by cyclic voltammetry. The detection scheme is based on competitive binding of salbutamol to the sensor surface whose differential pulse voltammetric signal decreases after competitive binding of the salbutamol-BSA conjugate and free salbutamol to the salbutamol antibody. The sensor responds to salbutamol in 5 to 150 nM concentration range, with a detection limit of 1 nM. This method was applied to the precise and sensitive determination of salbutamol in spiked feed samples. Figure In this work, we constructed a salbutamol immunosensor which was based on salbutamol-Ab adsorbed on the AuNPs/PTH/MWCNTs/GCE. Just as the procedures shown in Graph1, competitive immunoreaction was the experimental principle. The percentage of current response of the immunosensor was proportional to salbutamol concentrations in the range of 5-150nM. Springer-Verlag, 2011 Immunosensor nationallicence Polythionine nationallicence Multi-wall carbon nanotubes nationallicence Gold nanoparticles nationallicence Salbutamol nationallicence Mi Qin Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Wang Zheng Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Chai Chun Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Zhang Juan Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Zhao Bo School of Chemical and Environmental Science, Nanjing Normal University, 210097, Nanjing, China aut Chen Chang School of Chemical and Environmental Science, Nanjing Normal University, 210097, Nanjing, China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 459-467 0026-3672 173:3-4<459 2011 173 604 https://doi.org/10.1007/s00604-011-0572-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0572-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mi Qin Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Wang Zheng Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Chai Chun Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Juan Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Zhao Bo School of Chemical and Environmental Science, Nanjing Normal University, 210097, Nanjing, China aut NATIONALLICENCE 700 1- Chen Chang School of Chemical and Environmental Science, Nanjing Normal University, 210097, Nanjing, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 459-467 0026-3672 173:3-4<459 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer