caa a22 4500 445880600 CHVBK 20180317145541.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0575-6 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0575-6 Disposable electrochemical immunosensor for myeloperoxidase based on the indium tin oxide electrode modified with an ionic liquid composite film containing gold nanoparticles, poly(o-phenylenediamine) and carbon nanotubes [Elektronische Daten] [Bei Liu, Lingsong Lu, Qiong Li, Guoming Xie] A disposable electrochemical myeloperoxidase (MPO) immunosensor was fabricated based on the indium tin oxide electrode modified with a film composed of gold nanoparticles (AuNPs), poly(o-phenylenediamine), multi-walled carbon nanotubes and an ionic liquid. The composite film on the surface of the electrode was prepared by in situ electropolymerization using the ionic liquid as a supporting electrolyte. Negatively charged AuNPs were then adsorbed on the modified electrode via amine-gold affinity and to immobilize MPO antibody. Finally, bovine serum albumin was employed to block possible remaining active sites on the AuNPs. The modification of the electrode was studied by cyclic voltammetry and scanning electron microscopy. The factors affecting the performance of the immunosensor were investigated in detail using the hexacyanoferrate redox system. The sensor exhibited good response to MPO over two linear ranges (from 0.2 to 23.4 and from 23.4 to 300ng.mL−1), with a detection limit of 0.05ng.mL−1 (at an S/N of 3). Figure A disposable electrochemical immunosensor for myeloperoxidase based on the indium tin oxide electrode modified with an ionic liquid composite film composed of gold nanoparticles, poly(o-phenylenediamine) and carbon nanotubes. Springer-Verlag, 2011 Electrochemical immunosensor nationallicence Myeloperoxidase nationallicence Ionic liquid nationallicence Indium tin oxide electrode nationallicence Liu Bei Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut Lu Lingsong Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut Li Qiong Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut Xie Guoming Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 513-520 0026-3672 173:3-4<513 2011 173 604 https://doi.org/10.1007/s00604-011-0575-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0575-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Bei Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Lu Lingsong Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Li Qiong Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut NATIONALLICENCE 700 1- Xie Guoming Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, Chongqing, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 513-520 0026-3672 173:3-4<513 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer