caa a22 4500 445880651 CHVBK 20180317145542.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0567-6 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0567-6 Integrated micro/nano-fluidic system for mixing and preconcentration of dissolved proteins [Elektronische Daten] [Khalid Anwar, Taeheon Han, Samuel Yu, Sun Kim] An integrated micro/nano-fluidic system is presented for protein analysis. It is comprised of an integrated micromixer (IMM) and a preconcentrator with a separation column. The passive and planar type of IMM is based on an unbalanced split and the cross collision of the fluidic streams. The IMM can be easily fabricated and integrated to the microfluidic system. The preconcentrator has nanochannels formed by the electrical breakdown of polydimethylsiloxane (PDMS) membrane by applying a high electrical shock, but without any nano-lithography. The integrated microdevice was used for sample preparation (mixing with tagging molecules) and subsequent concentration of proteins. Proteins were electrokinetically trapped near the junction of the micro/nanochannels. We show a conceptual design and a simple microfluidic system for purposes of mixing and preconcentration. Figure Mixing and preconcentration of dissolved proteins using an integrated micro/nano-fluidic system Springer-Verlag, 2011 Micro/Nano-fluidic system nationallicence Micromixer nationallicence Protein preconcentration nationallicence Electrokinetic trapping nationallicence Anwar Khalid Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut Han Taeheon Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut Yu Samuel Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut Kim Sun Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 331-335 0026-3672 173:3-4<331 2011 173 604 https://doi.org/10.1007/s00604-011-0567-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0567-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Anwar Khalid Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Han Taeheon Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Yu Samuel Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Sun Department of Mechanical Engineering, Inha University, 402-751, Incheon, Republic of Korea aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 331-335 0026-3672 173:3-4<331 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer