caa a22 4500 445880678 CHVBK 20180317145542.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0569-4 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0569-4 Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells [Elektronische Daten] [Ewa Gorodkiewicz, Elżbieta Regulska, Kazimierz Wojtulewski] Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of an SPR sensor that was first modified with 1-octadecanethiol. A concentration of CGI-I equal to 4.0μg·mL-1 and a pH of 8.0 were found to give the best results. The dynamic response of the sensor ranges from 0.25 to 1.5ng·mL-1, and the detection limit is 0.12ng·mL-1. The sensor was applied to detect CatG in human saliva and white blood cells. Figure The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of cathepsin G via a newly developed surface plasmon resonance imaging assay. The sensor was applied to detect cathepsin G in human saliva and white blood cells. The Author(s), 2011 Biosensor nationallicence Cathepsin G nationallicence Cathepsin G inhibitor I nationallicence Surface plasmon resonance imaging nationallicence Gorodkiewicz Ewa Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, PL-15-443, Bialystok, Poland aut Regulska Elżbieta Department of General and Inorganic Chemistry, Institute of Chemistry, University of Bialystok, Hurtowa 1, PL-15-399, Bialystok, Poland aut Wojtulewski Kazimierz Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, PL-15-443, Bialystok, Poland aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 407-413 0026-3672 173:3-4<407 2011 173 604 https://doi.org/10.1007/s00604-011-0569-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0569-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gorodkiewicz Ewa Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, PL-15-443, Bialystok, Poland aut NATIONALLICENCE 700 1- Regulska Elżbieta Department of General and Inorganic Chemistry, Institute of Chemistry, University of Bialystok, Hurtowa 1, PL-15-399, Bialystok, Poland aut NATIONALLICENCE 700 1- Wojtulewski Kazimierz Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, PL-15-443, Bialystok, Poland aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 407-413 0026-3672 173:3-4<407 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer