caa a22 4500 445880724 CHVBK 20180317145542.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00604-011-0560-0 doi (NATIONALLICENCE)springer-10.1007/s00604-011-0560-0 Enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic assay for several (fluoro)quinolones in milk [Elektronische Daten] [Wei Sheng, Yuanzhen Li, Xin Xu, Meng Yuan, Shuo Wang] We have developed a heterologous direct competitive enzyme-linked immunosorbent assay (ELISA) and a visual colloidal gold-based immunochromatographic assay (CGIA) for simultaneous determination of ofloxacin, marbofloxacin, and fleroxacin residues in milk using polyclonal antibodies. The half-maximum inhibition concentrations (IC50) of ofloxacin, marbofloxacin, fleroxacin, and limits of detection (LODs; calculated as IC15 values) are between 0.20 and 0.53ng mL−1, and between 0.02 and 0.05ng mL−1, respectively. The average recoveries range from of 78% to 113%, and the coefficients of variation of intra- and inter-assays are between 2 and 11%, and 3 to 19%, respectively. The LODs for ofloxacin, marbofloxacin, fleroxacin in milk are between 3.5 and 8.9ng mL−1. The visual minimum detection limit of the optimized CGIA is 2ng mL−1 for milk samples. The detection process can be completed within 10min. The strips can be stored at 4°C for 8weeks without significant loss of activity. The results of the analysis of spiked samples showed that the CGIA can be applied to preliminary, fast, and on-site screening of milk samples. The ELISA and CGIA allow for a rapid, sensitive, and low-cost determination of (fluoro)quinolones residues in milk samples. Figure A direct competitive enzyme-linked immunosorbent assay (ELISA) and a visual colloidal gold-based immunochromatographic assay (CGIA) are proposed for simultaneous determination of ofloxacin, marbofloxacin, and fleroxacin residues in milk using polyclonal antibodies Springer-Verlag, 2011 (Fluoro)quinolones nationallicence Residue nationallicence ELISA nationallicence CGIA nationallicence Milk nationallicence Sheng Wei Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut Li Yuanzhen Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut Xu Xin Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut Yuan Meng Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut Wang Shuo Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 307-316 0026-3672 173:3-4<307 2011 173 604 https://doi.org/10.1007/s00604-011-0560-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0560-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sheng Wei Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Li Yuanzhen Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Xu Xin Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Yuan Meng Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Wang Shuo Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, 300457, Tianjin, China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/3-4(2011-06-01), 307-316 0026-3672 173:3-4<307 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer