caa a22 4500 445880813 CHVBK 20180317145542.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00604-011-0545-z doi (NATIONALLICENCE)springer-10.1007/s00604-011-0545-z Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA [Elektronische Daten] [Dalibor Huska, Vojtech Adam, Petr Babula, Libuse Trnkova, Jaromir Hubalek, Josef Zehnalek, Ladislav Havel, Rene Kizek] The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and poly-adenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles. Figa Microfluidic robotic device coupled with electrochemical sensor field Springer-Verlag, 2011 Electrochemical detection nationallicence Microfluidics nationallicence Environmental stress nationallicence Cadmium ions nationallicence Paramagnetic particles nationallicence Huska Dalibor Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut Adam Vojtech Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut Babula Petr Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42, Brno, Czech Republic aut Trnkova Libuse Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37, Brno, Czech Republic aut Hubalek Jaromir Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00, Brno, Czech Republic aut Zehnalek Josef Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut Havel Ladislav Department of Plant Biology, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut Kizek Rene Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 189-197 0026-3672 173:1-2<189 2011 173 604 https://doi.org/10.1007/s00604-011-0545-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-011-0545-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huska Dalibor Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Adam Vojtech Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Babula Petr Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42, Brno, Czech Republic aut NATIONALLICENCE 700 1- Trnkova Libuse Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37, Brno, Czech Republic aut NATIONALLICENCE 700 1- Hubalek Jaromir Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Zehnalek Josef Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Havel Ladislav Department of Plant Biology, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Kizek Rene Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, CZ-613 00, Brno, Czech Republic aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 189-197 0026-3672 173:1-2<189 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer