caa a22 4500 44588083X CHVBK 20180317145542.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00604-010-0529-4 doi (NATIONALLICENCE)springer-10.1007/s00604-010-0529-4 Direct electrochemistry of myoglobin based on electrodeposition of Pd nanoparticles with carbon ionic liquid electrode as basic electrode [Elektronische Daten] [Jinmei Huang, Jianbin Zheng, Qinglin Sheng] We report on the direct electrochemistry and electrocatalytic properties of myoglobin (Mb) immobilized on a carbon ionic liquid electrode covered with a matrix composed of an ionic liquid, gellan gum, and Pd nanoparticles. UV-vis and FT-IR spectroscopy confirm that Mb retains its native structure in the composite film on the electrode. Scanning electron microscopy reveals that the nanoparticles are deposited on the surface of the Pd electrode. Cyclic voltametry gives a pair of well-defined and quasireversible redox peaks with a formal potential (E 0′) of −332 mV and a peak-to-peak separation of 64 mV at near-neutral pH value. The modified electrode shows good electrocatalytic activity towards the reduction of hydrogen peroxide, with a linear range between 5.0 μM and 0.27 mM and a detection limit of 1.7 μM (S/N = 3). The apparent Michaelis-Menten constant is 88 μM. Figure A pair of well-defined redox peaks appeared on the cyclic voltammogram of Mb-GG-EMIMBF4/Pd/CILE (c) in pH 7.0 phosphate buffer saline at a scan rate of 500 mV·s−1 Springer-Verlag, 2011 Direct electrochemistry nationallicence Electrocatalysis nationallicence Electrodeposition nationallicence Myoglobin nationallicence Hydrogen peroxide nationallicence Huang Jinmei Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut Zheng Jianbin Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut Sheng Qinglin Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 157-163 0026-3672 173:1-2<157 2011 173 604 https://doi.org/10.1007/s00604-010-0529-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00604-010-0529-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Jinmei Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Jianbin Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 700 1- Sheng Qinglin Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, 710069, Xi'an, Shaanxi, People's Republic of China aut NATIONALLICENCE 773 0- Microchimica Acta Springer Vienna 173/1-2(2011-04-01), 157-163 0026-3672 173:1-2<157 2011 173 604 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer